Primer Details for TRINITY_DN13903_c4_g1_i1 | |||
|---|---|---|---|
| (AAT)7: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGAAGAGAATTCCCTGCCCC | CCACTAATGGCTTGTTTAGGTTT | Start: 227 |
| Tm | 60.106 | 57.278 | End: 376 |
| Size | 20 | 23 | 150 |
| Primer 2 details | |||
| Sequence | AGGTTTTTGCACCAATTACTTCA | AACCTGAATGGGAAAGGGGC | Start: 162 |
| Tm | 57.394 | 60.252 | End: 261 |
| Size | 23 | 20 | 100 |
| Primer 3 details | |||
| Sequence | GGAAGAGAATTCCCTGCCCC | AATGGCTTGTTTAGGTTTTGGAT | Start: 227 |
| Tm | 60.106 | 57.176 | End: 371 |
| Size | 20 | 23 | 145 |
| Primer 4 details | |||
| Sequence | GGAAGAGAATTCCCTGCCCC | ACTAATGGCTTGTTTAGGTTTTGGA | Start: 227 |
| Tm | 60.106 | 59.164 | End: 374 |
| Size | 20 | 25 | 148 |
| Primer 5 details | |||
| Sequence | GCCCCTTTCCCATTCAGGTT | CCACTAATGGCTTGTTTAGGTTT | Start: 242 |
| Tm | 60.252 | 57.278 | End: 376 |
| Size | 20 | 23 | 135 |
