ssr_primer
Primer Details for TRINITY_DN13903_c2_g1_i4 |
|---|
| (TAA)8tagagaaaaaagaaaggcatatttctcaaaagttgatcaaagatgggaccataccctatatattccattgcaaaataacacctactctttttttcttc(T)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACCCAACTGTGTTGCCTTGA | CTCCATCGAGATCCACAGGC | Start: 2646 |
| Tm | 60.034 | 59.967 | End: 2830 |
| Size | 20 | 20 | 185 |
| Primer 2 details |
|---|
| Sequence | CCTTCACACTGGAGGCTCTG | GCAACAAAACTCAGGGCTGG | Start: 4162 |
| Tm | 60.037 | 59.967 | End: 4285 |
| Size | 20 | 20 | 124 |
| Primer 3 details |
|---|
| Sequence | CGAACTCTGAGAGCTGTGCA | AGACTTTGGACGACCGACAC | Start: 3079 |
| Tm | 60.039 | 59.968 | End: 3206 |
| Size | 20 | 20 | 128 |
| Primer 4 details |
|---|
| Sequence | ACCCAACTGTGTTGCCTTGA | AGATCCACAGGCATTGCACA | Start: 2646 |
| Tm | 60.034 | 59.961 | End: 2822 |
| Size | 20 | 20 | 177 |
| Primer 5 details |
|---|
| Sequence | CCTTCACACTGGAGGCTCTG | TGAAGAAGCTCTGCCTGTGG | Start: 4162 |
| Tm | 60.037 | 59.964 | End: 4264 |
| Size | 20 | 20 | 103 |
(A)10ccaatc(AT)6tcccatttttgtataatttttgtgaataaattagttttttacccag(T)12: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACCCAACTGTGTTGCCTTGA | CTCCATCGAGATCCACAGGC | Start: 2646 |
| Tm | 60.034 | 59.967 | End: 2830 |
| Size | 20 | 20 | 185 |
| Primer 2 details |
|---|
| Sequence | CCTTCACACTGGAGGCTCTG | GCAACAAAACTCAGGGCTGG | Start: 4162 |
| Tm | 60.037 | 59.967 | End: 4285 |
| Size | 20 | 20 | 124 |
| Primer 3 details |
|---|
| Sequence | CGAACTCTGAGAGCTGTGCA | AGACTTTGGACGACCGACAC | Start: 3079 |
| Tm | 60.039 | 59.968 | End: 3206 |
| Size | 20 | 20 | 128 |
| Primer 4 details |
|---|
| Sequence | ACCCAACTGTGTTGCCTTGA | AGATCCACAGGCATTGCACA | Start: 2646 |
| Tm | 60.034 | 59.961 | End: 2822 |
| Size | 20 | 20 | 177 |
| Primer 5 details |
|---|
| Sequence | CCTTCACACTGGAGGCTCTG | TGAAGAAGCTCTGCCTGTGG | Start: 4162 |
| Tm | 60.037 | 59.964 | End: 4264 |
| Size | 20 | 20 | 103 |
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