ssr_primer
Primer Details for TRINITY_DN13898_c1_g9_i1 |
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| (T)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AAGTGCCGGAGAAGTCTTCG | GTCAAACACCAACACGCCTC | Start: 458 |
| Tm | 60.038 | 59.971 | End: 591 |
| Size | 20 | 20 | 134 |
| Primer 2 details |
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| Sequence | GAGGCGTGTTGGTGTTTGAC | GGAACGCCAGCTTTGTCAAG | Start: 572 |
| Tm | 59.971 | 60.04 | End: 746 |
| Size | 20 | 20 | 175 |
| Primer 3 details |
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| Sequence | ACACTCTTTCCCTACACGCG | TGGAGGAAGGCGTGGAGATA | Start: 35 |
| Tm | 60.039 | 60.033 | End: 179 |
| Size | 20 | 20 | 145 |
| Primer 4 details |
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| Sequence | CGCTCTCCTTTCCCTTCTGG | GTCAAACACCAACACGCCTC | Start: 386 |
| Tm | 60.108 | 59.971 | End: 591 |
| Size | 20 | 20 | 206 |
| Primer 5 details |
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| Sequence | TATCTCCACGCCTTCCTCCA | CCAGAAGGGAAAGGAGAGCG | Start: 160 |
| Tm | 60.033 | 60.108 | End: 405 |
| Size | 20 | 20 | 246 |
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