ssr_primer
Primer Details for TRINITY_DN13882_c2_g5_i18 |
|---|
| (TC)9: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GAGCTCTTCCCACTGCCATT | ACACAGCTTCGAAACGGCTA | Start: 114 |
| Tm | 60.035 | 59.967 | End: 297 |
| Size | 20 | 20 | 184 |
| Primer 2 details |
|---|
| Sequence | AAGATTTGTACGGCTGCGGA | CACAAGACCCTCGTCACCTC | Start: 614 |
| Tm | 60.037 | 60.038 | End: 861 |
| Size | 20 | 20 | 248 |
| Primer 3 details |
|---|
| Sequence | TCCTCCCATGGCCTTCCTAA | ATCAACGCCAGCATGAGTCA | Start: 1273 |
| Tm | 59.954 | 60.036 | End: 1450 |
| Size | 20 | 20 | 178 |
| Primer 4 details |
|---|
| Sequence | CCAAGCGATCGTTCGTTTCC | CACAAGACCCTCGTCACCTC | Start: 719 |
| Tm | 59.905 | 60.038 | End: 861 |
| Size | 20 | 20 | 143 |
| Primer 5 details |
|---|
| Sequence | CTGAGAGCTCTTCCCACTGC | ACACAGCTTCGAAACGGCTA | Start: 110 |
| Tm | 60.108 | 59.967 | End: 297 |
| Size | 20 | 20 | 188 |
(T)24: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GAGCTCTTCCCACTGCCATT | ACACAGCTTCGAAACGGCTA | Start: 114 |
| Tm | 60.035 | 59.967 | End: 297 |
| Size | 20 | 20 | 184 |
| Primer 2 details |
|---|
| Sequence | AAGATTTGTACGGCTGCGGA | CACAAGACCCTCGTCACCTC | Start: 614 |
| Tm | 60.037 | 60.038 | End: 861 |
| Size | 20 | 20 | 248 |
| Primer 3 details |
|---|
| Sequence | TCCTCCCATGGCCTTCCTAA | ATCAACGCCAGCATGAGTCA | Start: 1273 |
| Tm | 59.954 | 60.036 | End: 1450 |
| Size | 20 | 20 | 178 |
| Primer 4 details |
|---|
| Sequence | CCAAGCGATCGTTCGTTTCC | CACAAGACCCTCGTCACCTC | Start: 719 |
| Tm | 59.905 | 60.038 | End: 861 |
| Size | 20 | 20 | 143 |
| Primer 5 details |
|---|
| Sequence | CTGAGAGCTCTTCCCACTGC | ACACAGCTTCGAAACGGCTA | Start: 110 |
| Tm | 60.108 | 59.967 | End: 297 |
| Size | 20 | 20 | 188 |
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