ssr_primer
Primer Details for TRINITY_DN13877_c1_g1_i2 |
|---|
| (T)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGTTCGGATCTCTCTCCACG | CGAGTCCCGGCTATCAGTTC | Start: 1077 |
| Tm | 59.972 | 59.969 | End: 1245 |
| Size | 20 | 20 | 169 |
| Primer 2 details |
|---|
| Sequence | CGTTCGGATCTCTCTCCACG | ACCGGACGAGTACTCATGGA | Start: 1077 |
| Tm | 59.972 | 60.035 | End: 1207 |
| Size | 20 | 20 | 131 |
| Primer 3 details |
|---|
| Sequence | CGTTCGGATCTCTCTCCACG | TCCACCGGACGAGTACTCAT | Start: 1077 |
| Tm | 59.972 | 60.035 | End: 1210 |
| Size | 20 | 20 | 134 |
| Primer 4 details |
|---|
| Sequence | GAACTGATAGCCGGGACTCG | TGTCCTCTGCTATCACCGGA | Start: 1226 |
| Tm | 59.969 | 60.034 | End: 1487 |
| Size | 20 | 20 | 262 |
| Primer 5 details |
|---|
| Sequence | TCGGATCTCTCTCCACGTGT | CGAGTCCCGGCTATCAGTTC | Start: 1080 |
| Tm | 60.035 | 59.969 | End: 1245 |
| Size | 20 | 20 | 166 |
(T)12: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGTTCGGATCTCTCTCCACG | CGAGTCCCGGCTATCAGTTC | Start: 1077 |
| Tm | 59.972 | 59.969 | End: 1245 |
| Size | 20 | 20 | 169 |
| Primer 2 details |
|---|
| Sequence | CGTTCGGATCTCTCTCCACG | ACCGGACGAGTACTCATGGA | Start: 1077 |
| Tm | 59.972 | 60.035 | End: 1207 |
| Size | 20 | 20 | 131 |
| Primer 3 details |
|---|
| Sequence | CGTTCGGATCTCTCTCCACG | TCCACCGGACGAGTACTCAT | Start: 1077 |
| Tm | 59.972 | 60.035 | End: 1210 |
| Size | 20 | 20 | 134 |
| Primer 4 details |
|---|
| Sequence | GAACTGATAGCCGGGACTCG | TGTCCTCTGCTATCACCGGA | Start: 1226 |
| Tm | 59.969 | 60.034 | End: 1487 |
| Size | 20 | 20 | 262 |
| Primer 5 details |
|---|
| Sequence | TCGGATCTCTCTCCACGTGT | CGAGTCCCGGCTATCAGTTC | Start: 1080 |
| Tm | 60.035 | 59.969 | End: 1245 |
| Size | 20 | 20 | 166 |
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