ssr_primer
Primer Details for TRINITY_DN13872_c1_g5_i10 |
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| (AG)14: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AAGATTTCTGCTGGGCCTCC | AGCGTACTCGTTGCAGTTGA | Start: 519 |
| Tm | 60.034 | 59.968 | End: 758 |
| Size | 20 | 20 | 240 |
| Primer 2 details |
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| Sequence | ACCGAACTTCCTGCAACGAT | AGCGTACTCGTTGCAGTTGA | Start: 593 |
| Tm | 59.965 | 59.968 | End: 758 |
| Size | 20 | 20 | 166 |
| Primer 3 details |
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| Sequence | CCCCTGACTTCATGCCCTTT | GAACCCTTGGCAAAAGCTGG | Start: 1329 |
| Tm | 59.96 | 59.966 | End: 1440 |
| Size | 20 | 20 | 112 |
| Primer 4 details |
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| Sequence | CCCCTGACTTCATGCCCTTT | GGTGCTCCTGTCATCGAGAG | Start: 1329 |
| Tm | 59.96 | 59.898 | End: 1527 |
| Size | 20 | 20 | 199 |
| Primer 5 details |
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| Sequence | GTGGTTTATTGGGTGGGGGT | GGAGGCCCAGCAGAAATCTT | Start: 352 |
| Tm | 59.884 | 60.034 | End: 538 |
| Size | 20 | 20 | 187 |
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