ssr_primer
Primer Details for TRINITY_DN13872_c1_g1_i3 |
|---|
| (A)37: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GTTCCCACACAGCTCCATGA | AACCTTCTGCCACCGATCAG | Start: 679 |
| Tm | 59.963 | 60.036 | End: 862 |
| Size | 20 | 20 | 184 |
| Primer 2 details |
|---|
| Sequence | CAGCTCTCAGGCGGAAAAGA | TTGAAGGCAGCTCTAGGCAC | Start: 1354 |
| Tm | 60.037 | 60.036 | End: 1529 |
| Size | 20 | 20 | 176 |
| Primer 3 details |
|---|
| Sequence | CCCACACAGCTCCATGAAGT | AACCTTCTGCCACCGATCAG | Start: 682 |
| Tm | 59.963 | 60.036 | End: 862 |
| Size | 20 | 20 | 181 |
| Primer 4 details |
|---|
| Sequence | GTTCCCACACAGCTCCATGA | CTTACCGGGGATCATCGACG | Start: 679 |
| Tm | 59.963 | 60.039 | End: 883 |
| Size | 20 | 20 | 205 |
| Primer 5 details |
|---|
| Sequence | GTTCCCACACAGCTCCATGA | CGTCTTACCGGGGATCATCG | Start: 679 |
| Tm | 59.963 | 60.039 | End: 886 |
| Size | 20 | 20 | 208 |
(TTA)5ttctcctttgttgtttttgttggtttgaatttataaaggtgcagtgacttttttcatcttttttgaagagtagattcctcagagtgaagaaggg(A)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GTTCCCACACAGCTCCATGA | AACCTTCTGCCACCGATCAG | Start: 679 |
| Tm | 59.963 | 60.036 | End: 862 |
| Size | 20 | 20 | 184 |
| Primer 2 details |
|---|
| Sequence | CAGCTCTCAGGCGGAAAAGA | TTGAAGGCAGCTCTAGGCAC | Start: 1354 |
| Tm | 60.037 | 60.036 | End: 1529 |
| Size | 20 | 20 | 176 |
| Primer 3 details |
|---|
| Sequence | CCCACACAGCTCCATGAAGT | AACCTTCTGCCACCGATCAG | Start: 682 |
| Tm | 59.963 | 60.036 | End: 862 |
| Size | 20 | 20 | 181 |
| Primer 4 details |
|---|
| Sequence | GTTCCCACACAGCTCCATGA | CTTACCGGGGATCATCGACG | Start: 679 |
| Tm | 59.963 | 60.039 | End: 883 |
| Size | 20 | 20 | 205 |
| Primer 5 details |
|---|
| Sequence | GTTCCCACACAGCTCCATGA | CGTCTTACCGGGGATCATCG | Start: 679 |
| Tm | 59.963 | 60.039 | End: 886 |
| Size | 20 | 20 | 208 |
(T)11cccttttga(AATGCC)9: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GTTCCCACACAGCTCCATGA | AACCTTCTGCCACCGATCAG | Start: 679 |
| Tm | 59.963 | 60.036 | End: 862 |
| Size | 20 | 20 | 184 |
| Primer 2 details |
|---|
| Sequence | CAGCTCTCAGGCGGAAAAGA | TTGAAGGCAGCTCTAGGCAC | Start: 1354 |
| Tm | 60.037 | 60.036 | End: 1529 |
| Size | 20 | 20 | 176 |
| Primer 3 details |
|---|
| Sequence | CCCACACAGCTCCATGAAGT | AACCTTCTGCCACCGATCAG | Start: 682 |
| Tm | 59.963 | 60.036 | End: 862 |
| Size | 20 | 20 | 181 |
| Primer 4 details |
|---|
| Sequence | GTTCCCACACAGCTCCATGA | CTTACCGGGGATCATCGACG | Start: 679 |
| Tm | 59.963 | 60.039 | End: 883 |
| Size | 20 | 20 | 205 |
| Primer 5 details |
|---|
| Sequence | GTTCCCACACAGCTCCATGA | CGTCTTACCGGGGATCATCG | Start: 679 |
| Tm | 59.963 | 60.039 | End: 886 |
| Size | 20 | 20 | 208 |
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