ssr_primer
Primer Details for TRINITY_DN13866_c1_g1_i4 |
|---|
| (T)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGCATCCTCATTGGTGCTGT | GTCCTGCCGCTCTCTCATAC | Start: 2399 |
| Tm | 59.961 | 59.969 | End: 2676 |
| Size | 20 | 20 | 278 |
| Primer 2 details |
|---|
| Sequence | TTTTCCACTTGCTGCTGTGC | GTCCTGCCGCTCTCTCATAC | Start: 2535 |
| Tm | 59.897 | 59.969 | End: 2676 |
| Size | 20 | 20 | 142 |
| Primer 3 details |
|---|
| Sequence | TGTGTGGCTTTTTCCCCCAT | GTCCTGCCGCTCTCTCATAC | Start: 2488 |
| Tm | 60.105 | 59.969 | End: 2676 |
| Size | 20 | 20 | 189 |
| Primer 4 details |
|---|
| Sequence | ATGTGTGGCTTTTTCCCCCA | GTCCTGCCGCTCTCTCATAC | Start: 2487 |
| Tm | 60.105 | 59.969 | End: 2676 |
| Size | 20 | 20 | 190 |
| Primer 5 details |
|---|
| Sequence | TTTTCCACTTGCTGCTGTGC | AAGAAAGTCCTGCCGCTCTC | Start: 2535 |
| Tm | 59.897 | 60.037 | End: 2682 |
| Size | 20 | 20 | 148 |
(T)11actttagagagcttatggctgaaaaggaacggaggtgttttgtatgagagagcggcaggactttcttgggtgtgaaaaggagaggatg(TA)6: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGCATCCTCATTGGTGCTGT | GTCCTGCCGCTCTCTCATAC | Start: 2399 |
| Tm | 59.961 | 59.969 | End: 2676 |
| Size | 20 | 20 | 278 |
| Primer 2 details |
|---|
| Sequence | TTTTCCACTTGCTGCTGTGC | GTCCTGCCGCTCTCTCATAC | Start: 2535 |
| Tm | 59.897 | 59.969 | End: 2676 |
| Size | 20 | 20 | 142 |
| Primer 3 details |
|---|
| Sequence | TGTGTGGCTTTTTCCCCCAT | GTCCTGCCGCTCTCTCATAC | Start: 2488 |
| Tm | 60.105 | 59.969 | End: 2676 |
| Size | 20 | 20 | 189 |
| Primer 4 details |
|---|
| Sequence | ATGTGTGGCTTTTTCCCCCA | GTCCTGCCGCTCTCTCATAC | Start: 2487 |
| Tm | 60.105 | 59.969 | End: 2676 |
| Size | 20 | 20 | 190 |
| Primer 5 details |
|---|
| Sequence | TTTTCCACTTGCTGCTGTGC | AAGAAAGTCCTGCCGCTCTC | Start: 2535 |
| Tm | 59.897 | 60.037 | End: 2682 |
| Size | 20 | 20 | 148 |
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