Primer Details for TRINITY_DN13859_c2_g5_i1 | |||
|---|---|---|---|
| (AT)10: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CAATGCTCAATCACAAGGTGGT | TCAAGTGTACTGGAGTAGGTAAAA | Start: 156 |
| Tm | 59.44 | 57.018 | End: 256 |
| Size | 22 | 24 | 101 |
| Primer 2 details | |||
| Sequence | ACAATGCTCAATCACAAGGTGG | TCAAGTGTACTGGAGTAGGTAAAA | Start: 155 |
| Tm | 59.44 | 57.018 | End: 256 |
| Size | 22 | 24 | 102 |
| Primer 3 details | |||
| Sequence | AGTTCTTGAAAATAAATGTTGTGTTGT | ACAACGCAAACCACCTTGTG | Start: 39 |
| Tm | 57.348 | 59.828 | End: 186 |
| Size | 27 | 20 | 148 |
| Primer 4 details | |||
| Sequence | AATGCTCAATCACAAGGTGGT | TCAAGTGTACTGGAGTAGGTAAAA | Start: 157 |
| Tm | 58.12 | 57.018 | End: 256 |
| Size | 21 | 24 | 100 |
| Primer 5 details | |||
| Sequence | CAATGCTCAATCACAAGGTGGTT | TCAAGTGTACTGGAGTAGGTAAAA | Start: 156 |
| Tm | 59.994 | 57.018 | End: 256 |
| Size | 23 | 24 | 101 |
