Primer Details for TRINITY_DN13855_c3_g1_i1 | |||
|---|---|---|---|
| (AAG)5: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGAAGAAGGGAATATTGGACGATGT | GCCTTTGGCTCTCCACAAAA | Start: 77 |
| Tm | 59.869 | 58.96 | End: 228 |
| Size | 25 | 20 | 152 |
| Primer 2 details | |||
| Sequence | GAAGAAGGGAATATTGGACGATGT | GCCTTTGGCTCTCCACAAAA | Start: 78 |
| Tm | 58.631 | 58.96 | End: 228 |
| Size | 24 | 20 | 151 |
| Primer 3 details | |||
| Sequence | AGAAGGGAATATTGGACGATGTT | GCCTTTGGCTCTCCACAAAA | Start: 80 |
| Tm | 57.626 | 58.96 | End: 228 |
| Size | 23 | 20 | 149 |
| Primer 4 details | |||
| Sequence | AAGAAGGGAATATTGGACGATGT | GCCTTTGGCTCTCCACAAAA | Start: 79 |
| Tm | 57.626 | 58.96 | End: 228 |
| Size | 23 | 20 | 150 |
| Primer 5 details | |||
| Sequence | AGAAGAAGAAGGGAATATTGGACGA | GCCTTTGGCTCTCCACAAAA | Start: 74 |
| Tm | 59.574 | 58.96 | End: 228 |
| Size | 25 | 20 | 155 |
