ssr_primer
Primer Details for TRINITY_DN13853_c0_g7_i11 |
|---|
| (T)12: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ATACGAGCATGGGCGTACAG | TGCAGAATGGACTGAGGCAG | Start: 671 |
| Tm | 59.968 | 60.036 | End: 844 |
| Size | 20 | 20 | 174 |
| Primer 2 details |
|---|
| Sequence | ATACGAGCATGGGCGTACAG | CTGCAGAATGGACTGAGGCA | Start: 671 |
| Tm | 59.968 | 60.036 | End: 845 |
| Size | 20 | 20 | 175 |
| Primer 3 details |
|---|
| Sequence | ATTGGTGCAAGCTTCCTCGA | CTGTACGCCCATGCTCGTAT | Start: 529 |
| Tm | 59.963 | 59.968 | End: 690 |
| Size | 20 | 20 | 162 |
| Primer 4 details |
|---|
| Sequence | AAGCTTCCTCGACAACCTCG | CTGTACGCCCATGCTCGTAT | Start: 537 |
| Tm | 60.038 | 59.968 | End: 690 |
| Size | 20 | 20 | 154 |
| Primer 5 details |
|---|
| Sequence | TGCCTCAGTCCATTCTGCAG | GCTGGCTTCCACTTGAGGAT | Start: 826 |
| Tm | 60.036 | 60.035 | End: 1022 |
| Size | 20 | 20 | 197 |
(A)17: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ATACGAGCATGGGCGTACAG | TGCAGAATGGACTGAGGCAG | Start: 671 |
| Tm | 59.968 | 60.036 | End: 844 |
| Size | 20 | 20 | 174 |
| Primer 2 details |
|---|
| Sequence | ATACGAGCATGGGCGTACAG | CTGCAGAATGGACTGAGGCA | Start: 671 |
| Tm | 59.968 | 60.036 | End: 845 |
| Size | 20 | 20 | 175 |
| Primer 3 details |
|---|
| Sequence | ATTGGTGCAAGCTTCCTCGA | CTGTACGCCCATGCTCGTAT | Start: 529 |
| Tm | 59.963 | 59.968 | End: 690 |
| Size | 20 | 20 | 162 |
| Primer 4 details |
|---|
| Sequence | AAGCTTCCTCGACAACCTCG | CTGTACGCCCATGCTCGTAT | Start: 537 |
| Tm | 60.038 | 59.968 | End: 690 |
| Size | 20 | 20 | 154 |
| Primer 5 details |
|---|
| Sequence | TGCCTCAGTCCATTCTGCAG | GCTGGCTTCCACTTGAGGAT | Start: 826 |
| Tm | 60.036 | 60.035 | End: 1022 |
| Size | 20 | 20 | 197 |
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