ssr_primer
Primer Details for TRINITY_DN13822_c2_g1_i8 |
|---|
| (A)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTACGTGTCGGTTGTTGGGA | CGAGGTCGATGTACAGGAGC | Start: 795 |
| Tm | 59.968 | 59.97 | End: 1011 |
| Size | 20 | 20 | 217 |
| Primer 2 details |
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| Sequence | GCTCCTGTACATCGACCTCG | TCCATGTCATCACCCACTGC | Start: 992 |
| Tm | 59.97 | 60.035 | End: 1196 |
| Size | 20 | 20 | 205 |
| Primer 3 details |
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| Sequence | TTGGAGACTGGGTTCGCATC | ACACCCGATCCCCAATCCTA | Start: 5022 |
| Tm | 60.036 | 60.031 | End: 5225 |
| Size | 20 | 20 | 204 |
| Primer 4 details |
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| Sequence | TTGGTCATCTGCATCGCCTT | ATGTCGACCTTAGCCTCCCT | Start: 3259 |
| Tm | 60.035 | 60.032 | End: 3472 |
| Size | 20 | 20 | 214 |
| Primer 5 details |
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| Sequence | AGGGAGGCTAAGGTCGACAT | GATCAGCTCCAGCCAACAGT | Start: 3453 |
| Tm | 60.032 | 60.036 | End: 3669 |
| Size | 20 | 20 | 217 |
(T)12: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTACGTGTCGGTTGTTGGGA | CGAGGTCGATGTACAGGAGC | Start: 795 |
| Tm | 59.968 | 59.97 | End: 1011 |
| Size | 20 | 20 | 217 |
| Primer 2 details |
|---|
| Sequence | GCTCCTGTACATCGACCTCG | TCCATGTCATCACCCACTGC | Start: 992 |
| Tm | 59.97 | 60.035 | End: 1196 |
| Size | 20 | 20 | 205 |
| Primer 3 details |
|---|
| Sequence | TTGGAGACTGGGTTCGCATC | ACACCCGATCCCCAATCCTA | Start: 5022 |
| Tm | 60.036 | 60.031 | End: 5225 |
| Size | 20 | 20 | 204 |
| Primer 4 details |
|---|
| Sequence | TTGGTCATCTGCATCGCCTT | ATGTCGACCTTAGCCTCCCT | Start: 3259 |
| Tm | 60.035 | 60.032 | End: 3472 |
| Size | 20 | 20 | 214 |
| Primer 5 details |
|---|
| Sequence | AGGGAGGCTAAGGTCGACAT | GATCAGCTCCAGCCAACAGT | Start: 3453 |
| Tm | 60.032 | 60.036 | End: 3669 |
| Size | 20 | 20 | 217 |
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