ssr_primer
Primer Details for TRINITY_DN13821_c2_g6_i4 |
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| (A)11ttgtgcgtatt(A)12: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGAAGAAGTGGTGCTGGACG | TCGATGGTATGGGGTGGAGT | Start: 135 |
| Tm | 59.966 | 60.032 | End: 371 |
| Size | 20 | 20 | 237 |
| Primer 2 details |
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| Sequence | ACTCCACCCCATACCATCGA | CTAAGGGTAGCAACGGCACA | Start: 352 |
| Tm | 60.032 | 60.037 | End: 516 |
| Size | 20 | 20 | 165 |
| Primer 3 details |
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| Sequence | TGGTGCTGGACGGCTATTTT | TCGATGGTATGGGGTGGAGT | Start: 143 |
| Tm | 59.962 | 60.032 | End: 371 |
| Size | 20 | 20 | 229 |
| Primer 4 details |
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| Sequence | CACCCCCAGAAGCAGCAATA | TGGCAAATCCCTTCTTCCCC | Start: 2023 |
| Tm | 60.034 | 59.959 | End: 2204 |
| Size | 20 | 20 | 182 |
| Primer 5 details |
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| Sequence | CACCCCCAGAAGCAGCAATA | TGGGTTCCTACGGGGAGAAT | Start: 2023 |
| Tm | 60.034 | 59.956 | End: 2247 |
| Size | 20 | 20 | 225 |
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