Primer Details for TRINITY_DN13814_c0_g4_i1 | |||
|---|---|---|---|
| (A)11: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACAAATTCCATTTTGGGGAGAAA | TGCCTTTTGTCACCCACTGT | Start: 12 |
| Tm | 57.097 | 60.034 | End: 119 |
| Size | 23 | 20 | 108 |
| Primer 2 details | |||
| Sequence | AACAAATTCCATTTTGGGGAGAA | TGCCTTTTGTCACCCACTGT | Start: 11 |
| Tm | 57.097 | 60.034 | End: 119 |
| Size | 23 | 20 | 109 |
| Primer 3 details | |||
| Sequence | AAACAAATTCCATTTTGGGGAGA | TGCCTTTTGTCACCCACTGT | Start: 10 |
| Tm | 57.097 | 60.034 | End: 119 |
| Size | 23 | 20 | 110 |
| Primer 4 details | |||
| Sequence | ACAAATTCCATTTTGGGGAGAAAAGA | TGCCTTTTGTCACCCACTGT | Start: 12 |
| Tm | 59.899 | 60.034 | End: 119 |
| Size | 26 | 20 | 108 |
| Primer 5 details | |||
| Sequence | ACAAATTCCATTTTGGGGAGAAA | GCTTGCCTTTTGTCACCCAC | Start: 12 |
| Tm | 57.097 | 60.249 | End: 122 |
| Size | 23 | 20 | 111 |
