Primer Details for TRINITY_DN13791_c10_g6_i1 |
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| (A)12: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTGGACATTACTCATTTGTTTTGGC | TCAGGAGGTGTAGCTGAGCA | Start: 50 |
| Tm | 59.011 | 60.252 | End: 149 |
| Size | 25 | 20 | 100 |
| Primer 2 details |
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| Sequence | TGGACATTACTCATTTGTTTTGGCT | CCATCGATCAGGAGGTGTAGC | Start: 51 |
| Tm | 59.696 | 60 | End: 156 |
| Size | 25 | 21 | 106 |
| Primer 3 details |
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| Sequence | TGGACATTACTCATTTGTTTTGGCT | TCGATCAGGAGGTGTAGCTGA | Start: 51 |
| Tm | 59.696 | 60.065 | End: 153 |
| Size | 25 | 21 | 103 |
| Primer 4 details |
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| Sequence | TGGACATTACTCATTTGTTTTGGCT | GATCAGGAGGTGTAGCTGAGC | Start: 51 |
| Tm | 59.696 | 59.932 | End: 151 |
| Size | 25 | 21 | 101 |
| Primer 5 details |
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| Sequence | TGGACATTACTCATTTGTTTTGGCT | ATCAGGAGGTGTAGCTGAGC | Start: 51 |
| Tm | 59.696 | 58.881 | End: 150 |
| Size | 25 | 20 | 100 |
