ssr_primer
Primer Details for TRINITY_DN13775_c3_g3_i4 |
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| (T)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ATTGGGAAGGGTAGGCAAGC | TCTTCCAGGGCAGCTTTAGC | Start: 1482 |
| Tm | 60.033 | 60.035 | End: 1723 |
| Size | 20 | 20 | 242 |
| Primer 2 details |
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| Sequence | CCTGTCGGCAAGGGGTTATT | TCTTCCAGGGCAGCTTTAGC | Start: 1543 |
| Tm | 60.034 | 60.035 | End: 1723 |
| Size | 20 | 20 | 181 |
| Primer 3 details |
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| Sequence | AGCATTGGCACAGGTTACGA | TAAGAGACACCCAACCGCAC | Start: 173 |
| Tm | 59.964 | 59.966 | End: 391 |
| Size | 20 | 20 | 219 |
| Primer 4 details |
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| Sequence | AGCATTGGCACAGGTTACGA | GTAAGAGACACCCAACCGCA | Start: 173 |
| Tm | 59.964 | 59.966 | End: 392 |
| Size | 20 | 20 | 220 |
| Primer 5 details |
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| Sequence | TTTCCAGATCGAGTACGCCG | TAAGAGACACCCAACCGCAC | Start: 229 |
| Tm | 59.9 | 59.966 | End: 391 |
| Size | 20 | 20 | 163 |
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