ssr_primer
Primer Details for TRINITY_DN13772_c0_g5_i1 |
|---|
| (T)13: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGGTATGAGGACCGTGACCT | AGGGTCCACACCTTGCAATT | Start: 175 |
| Tm | 59.665 | 59.812 | End: 315 |
| Size | 20 | 20 | 141 |
| Primer 2 details |
|---|
| Sequence | AATTGCAAGGTGTGGACCCT | GCCCAAAAGAGAACTCCGCT | Start: 296 |
| Tm | 59.812 | 60.607 | End: 554 |
| Size | 20 | 20 | 259 |
| Primer 3 details |
|---|
| Sequence | AATTGCAAGGTGTGGACCCT | AGCCCAAAAGAGAACTCCGC | Start: 296 |
| Tm | 59.812 | 60.607 | End: 555 |
| Size | 20 | 20 | 260 |
| Primer 4 details |
|---|
| Sequence | GCGGAGTTCTCTTTTGGGCT | ACTGGCTTCCTTTGCGTCAT | Start: 536 |
| Tm | 60.607 | 60.251 | End: 792 |
| Size | 20 | 20 | 257 |
| Primer 5 details |
|---|
| Sequence | AGCGGAGTTCTCTTTTGGGC | ACTGGCTTCCTTTGCGTCAT | Start: 535 |
| Tm | 60.607 | 60.251 | End: 792 |
| Size | 20 | 20 | 258 |
(T)10cttcaaaaaaatgtgtcgttttatgtttagcggagttctcttttgggc(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGGTATGAGGACCGTGACCT | AGGGTCCACACCTTGCAATT | Start: 175 |
| Tm | 59.665 | 59.812 | End: 315 |
| Size | 20 | 20 | 141 |
| Primer 2 details |
|---|
| Sequence | AATTGCAAGGTGTGGACCCT | GCCCAAAAGAGAACTCCGCT | Start: 296 |
| Tm | 59.812 | 60.607 | End: 554 |
| Size | 20 | 20 | 259 |
| Primer 3 details |
|---|
| Sequence | AATTGCAAGGTGTGGACCCT | AGCCCAAAAGAGAACTCCGC | Start: 296 |
| Tm | 59.812 | 60.607 | End: 555 |
| Size | 20 | 20 | 260 |
| Primer 4 details |
|---|
| Sequence | GCGGAGTTCTCTTTTGGGCT | ACTGGCTTCCTTTGCGTCAT | Start: 536 |
| Tm | 60.607 | 60.251 | End: 792 |
| Size | 20 | 20 | 257 |
| Primer 5 details |
|---|
| Sequence | AGCGGAGTTCTCTTTTGGGC | ACTGGCTTCCTTTGCGTCAT | Start: 535 |
| Tm | 60.607 | 60.251 | End: 792 |
| Size | 20 | 20 | 258 |
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