Primer Details for TRINITY_DN13765_c0_g1_i1 |
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| (T)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGATGAGATTTGAACTCACAACTGA | ACTCGGACATGAACGGATCT | Start: 74 |
| Tm | 58.472 | 58.525 | End: 225 |
| Size | 25 | 20 | 152 |
| Primer 2 details |
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| Sequence | GATGAGATTTGAACTCACAACTGA | ACTCGGACATGAACGGATCT | Start: 75 |
| Tm | 57.208 | 58.525 | End: 225 |
| Size | 24 | 20 | 151 |
| Primer 3 details |
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| Sequence | AGATGAGATTTGAACTCACAACTG | ACTCGGACATGAACGGATCT | Start: 74 |
| Tm | 57.202 | 58.525 | End: 225 |
| Size | 24 | 20 | 152 |
| Primer 4 details |
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| Sequence | AGATGAGATTTGAACTCACAACTGA | AGGGACACGTGTTCTCTTTCA | Start: 74 |
| Tm | 58.472 | 59.239 | End: 194 |
| Size | 25 | 21 | 121 |
| Primer 5 details |
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| Sequence | AGATGAGATTTGAACTCACAACTGA | AACTCGGACATGAACGGATCT | Start: 74 |
| Tm | 58.472 | 59.175 | End: 226 |
| Size | 25 | 21 | 153 |
