Primer Details for TRINITY_DN13760_c1_g1_i1 |
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| (A)11: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGGGTTTGAAGTTTCTTAATATGGTCT | AGATTTCTAGCTGGCCGAACT | Start: 38 |
| Tm | 59.052 | 59.166 | End: 177 |
| Size | 27 | 21 | 140 |
| Primer 2 details |
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| Sequence | TGGGTTTGAAGTTTCTTAATATGGTC | AGATTTCTAGCTGGCCGAACT | Start: 38 |
| Tm | 57.882 | 59.166 | End: 177 |
| Size | 26 | 21 | 140 |
| Primer 3 details |
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| Sequence | GGGTTTGAAGTTTCTTAATATGGTCTG | AGATTTCTAGCTGGCCGAACT | Start: 39 |
| Tm | 58.756 | 59.166 | End: 177 |
| Size | 27 | 21 | 139 |
| Primer 4 details |
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| Sequence | GGGTTTGAAGTTTCTTAATATGGTCT | AGATTTCTAGCTGGCCGAACT | Start: 39 |
| Tm | 57.65 | 59.166 | End: 177 |
| Size | 26 | 21 | 139 |
| Primer 5 details |
|---|
| Sequence | TGGGTTTGAAGTTTCTTAATATGGTCT | AGATTTCTAGCTGGCCGAACTT | Start: 38 |
| Tm | 59.052 | 59.763 | End: 177 |
| Size | 27 | 22 | 140 |
