ssr_primer
Primer Details for TRINITY_DN13731_c1_g1_i1 |
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| (TTCT)5: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTGAAGCTCTTCCTGCCCAG | TGCGGGGGACTTCAATTGAG | Start: 160 |
| Tm | 59.963 | 60.322 | End: 319 |
| Size | 20 | 20 | 160 |
| Primer 2 details |
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| Sequence | TTGAAGCTCTTCCTGCCCAG | CTGCGGGGGACTTCAATTGA | Start: 160 |
| Tm | 59.963 | 60.322 | End: 320 |
| Size | 20 | 20 | 161 |
| Primer 3 details |
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| Sequence | TTGAAGCTCTTCCTGCCCAG | GCGGGGGACTTCAATTGAGT | Start: 160 |
| Tm | 59.963 | 60.323 | End: 318 |
| Size | 20 | 20 | 159 |
| Primer 4 details |
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| Sequence | TTGAAGCTCTTCCTGCCCAG | TAATGGAAAGGGACACGGCG | Start: 160 |
| Tm | 59.963 | 60.392 | End: 418 |
| Size | 20 | 20 | 259 |
| Primer 5 details |
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| Sequence | AACTTGGACAGTTCACGCCA | TAATGGAAAGGGACACGGCG | Start: 280 |
| Tm | 60.107 | 60.392 | End: 418 |
| Size | 20 | 20 | 139 |
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