Primer Details for TRINITY_DN13730_c7_g5_i1 |
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| (A)12: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACTGAAAAACAAACTAATCCTAATCCT | AGGGTATTGTGGTCAAATCCTT | Start: 42 |
| Tm | 57.412 | 57.325 | End: 172 |
| Size | 27 | 22 | 131 |
| Primer 2 details |
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| Sequence | ACTGAAAAACAAACTAATCCTAATCCT | TGGGAGGGATAGAAATGATGTAGT | Start: 42 |
| Tm | 57.412 | 58.523 | End: 202 |
| Size | 27 | 24 | 161 |
| Primer 3 details |
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| Sequence | ACTGAAAAACAAACTAATCCTAATCCT | AGTAGGAATAGGGTATTGTGGTCA | Start: 42 |
| Tm | 57.412 | 58.465 | End: 181 |
| Size | 27 | 24 | 140 |
| Primer 4 details |
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| Sequence | ACTGAAAAACAAACTAATCCTAATCCT | GGAATAGGGTATTGTGGTCAAATCC | Start: 42 |
| Tm | 57.412 | 59.465 | End: 177 |
| Size | 27 | 25 | 136 |
| Primer 5 details |
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| Sequence | ACTGAAAAACAAACTAATCCTAATCCT | TTTGGGAGGGATAGAAATGATGT | Start: 42 |
| Tm | 57.412 | 57.268 | End: 204 |
| Size | 27 | 23 | 163 |
