ssr_primer
Primer Details for TRINITY_DN13727_c0_g5_i1 |
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| (TC)6: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCTCTCTTCCGTTCCGTCCT | AACCGGGTTGAATGGGACTC | Start: 538 |
| Tm | 59.962 | 59.962 | End: 745 |
| Size | 20 | 20 | 208 |
| Primer 2 details |
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| Sequence | TCTCTCTTCCGTTCCGTCCT | GTGGATGATGGGCTGCCTTA | Start: 538 |
| Tm | 59.962 | 59.817 | End: 787 |
| Size | 20 | 20 | 250 |
| Primer 3 details |
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| Sequence | TTGCAACTGAAGGTGGTGGT | TCCTACTCCTCAGTCCGCAT | Start: 1593 |
| Tm | 60.034 | 59.741 | End: 1692 |
| Size | 20 | 20 | 100 |
| Primer 4 details |
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| Sequence | TGCTCCATTTCCACCCTCTG | AACCGGGTTGAATGGGACTC | Start: 602 |
| Tm | 59.671 | 59.962 | End: 745 |
| Size | 20 | 20 | 144 |
| Primer 5 details |
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| Sequence | TTGGGTTTTAAGGCAGCCCA | GCTGGCCGAAAGACTCAGAA | Start: 760 |
| Tm | 60.105 | 60.321 | End: 1030 |
| Size | 20 | 20 | 271 |
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