ssr_primer
Primer Details for TRINITY_DN13724_c3_g5_i1 |
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| (A)21: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGAACATAAGCGGACACCCA | TGCTTTCCGTGCATGTTCCT | Start: 294 |
| Tm | 59.312 | 60.537 | End: 479 |
| Size | 20 | 20 | 186 |
| Primer 2 details |
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| Sequence | TGAACATAAGCGGACACCCA | TGGGGGAAGCAATTCTCACA | Start: 294 |
| Tm | 59.312 | 59.223 | End: 450 |
| Size | 20 | 20 | 157 |
| Primer 3 details |
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| Sequence | TGAACATAAGCGGACACCCA | GGGGAAGCAATTCTCACAGC | Start: 294 |
| Tm | 59.312 | 59.186 | End: 448 |
| Size | 20 | 20 | 155 |
| Primer 4 details |
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| Sequence | TGAACATAAGCGGACACCCAA | TGCTTTCCGTGCATGTTCCT | Start: 294 |
| Tm | 59.928 | 60.537 | End: 479 |
| Size | 21 | 20 | 186 |
| Primer 5 details |
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| Sequence | TGGGGGAAATAGGGTTGCTT | TGGGTGTCCGCTTATGTTCA | Start: 117 |
| Tm | 58.911 | 59.312 | End: 313 |
| Size | 20 | 20 | 197 |
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