ssr_primer
Primer Details for TRINITY_DN13724_c3_g4_i1 |
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| (T)14: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGAAAATACGCTTGCCGGAG | CTCTCCTCTCTCCCTTCCCC | Start: 117 |
| Tm | 59.974 | 60.106 | End: 217 |
| Size | 20 | 20 | 101 |
| Primer 2 details |
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| Sequence | CGAAAATACGCTTGCCGGAG | ACCCTCTCTCCTCTCTCCCT | Start: 117 |
| Tm | 59.974 | 60.254 | End: 222 |
| Size | 20 | 20 | 106 |
| Primer 3 details |
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| Sequence | GTCCGAAAATACGCTTGCCG | CTCTCCTCTCTCCCTTCCCC | Start: 114 |
| Tm | 60.248 | 60.106 | End: 217 |
| Size | 20 | 20 | 104 |
| Primer 4 details |
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| Sequence | ATACGCTTGCCGGAGAGAAG | ACCCTCTCTCCTCTCTCCCT | Start: 122 |
| Tm | 59.897 | 60.254 | End: 222 |
| Size | 20 | 20 | 101 |
| Primer 5 details |
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| Sequence | GGGGAAGGGAGAGAGGAGAG | CTTGACCATTACCCCCGGTG | Start: 198 |
| Tm | 60.106 | 60.394 | End: 443 |
| Size | 20 | 20 | 246 |
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