ssr_primer
Primer Details for TRINITY_DN13721_c1_g3_i1 |
|---|
| (T)11: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTGTCCCAAGTATGGCGGTC | TGGGGAGATTGCTCGAGGTA | Start: 1528 |
| Tm | 60.036 | 60.033 | End: 1730 |
| Size | 20 | 20 | 203 |
| Primer 2 details |
|---|
| Sequence | TCTCGACAACCCTTTGGAGC | CCGTACACACATGGACCCAA | Start: 2001 |
| Tm | 59.965 | 59.964 | End: 2129 |
| Size | 20 | 20 | 129 |
| Primer 3 details |
|---|
| Sequence | ACGTGGACCCCAAAGTTGTT | TTGGGGTCCTTCTTCCAGGA | Start: 3042 |
| Tm | 60.034 | 60.104 | End: 3245 |
| Size | 20 | 20 | 204 |
| Primer 4 details |
|---|
| Sequence | TCCTGGAAGAAGGACCCCAA | CAGCAGCAGCAGCATCAAAA | Start: 3226 |
| Tm | 60.104 | 60.039 | End: 3376 |
| Size | 20 | 20 | 151 |
| Primer 5 details |
|---|
| Sequence | ACGTGGACCCCAAAGTTGTT | CTTTTCGTCAGCTTCCGCAC | Start: 3042 |
| Tm | 60.034 | 60.111 | End: 3206 |
| Size | 20 | 20 | 165 |
(CAT)5: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTGTCCCAAGTATGGCGGTC | TGGGGAGATTGCTCGAGGTA | Start: 1528 |
| Tm | 60.036 | 60.033 | End: 1730 |
| Size | 20 | 20 | 203 |
| Primer 2 details |
|---|
| Sequence | TCTCGACAACCCTTTGGAGC | CCGTACACACATGGACCCAA | Start: 2001 |
| Tm | 59.965 | 59.964 | End: 2129 |
| Size | 20 | 20 | 129 |
| Primer 3 details |
|---|
| Sequence | ACGTGGACCCCAAAGTTGTT | TTGGGGTCCTTCTTCCAGGA | Start: 3042 |
| Tm | 60.034 | 60.104 | End: 3245 |
| Size | 20 | 20 | 204 |
| Primer 4 details |
|---|
| Sequence | TCCTGGAAGAAGGACCCCAA | CAGCAGCAGCAGCATCAAAA | Start: 3226 |
| Tm | 60.104 | 60.039 | End: 3376 |
| Size | 20 | 20 | 151 |
| Primer 5 details |
|---|
| Sequence | ACGTGGACCCCAAAGTTGTT | CTTTTCGTCAGCTTCCGCAC | Start: 3042 |
| Tm | 60.034 | 60.111 | End: 3206 |
| Size | 20 | 20 | 165 |
(TGC)5: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTGTCCCAAGTATGGCGGTC | TGGGGAGATTGCTCGAGGTA | Start: 1528 |
| Tm | 60.036 | 60.033 | End: 1730 |
| Size | 20 | 20 | 203 |
| Primer 2 details |
|---|
| Sequence | TCTCGACAACCCTTTGGAGC | CCGTACACACATGGACCCAA | Start: 2001 |
| Tm | 59.965 | 59.964 | End: 2129 |
| Size | 20 | 20 | 129 |
| Primer 3 details |
|---|
| Sequence | ACGTGGACCCCAAAGTTGTT | TTGGGGTCCTTCTTCCAGGA | Start: 3042 |
| Tm | 60.034 | 60.104 | End: 3245 |
| Size | 20 | 20 | 204 |
| Primer 4 details |
|---|
| Sequence | TCCTGGAAGAAGGACCCCAA | CAGCAGCAGCAGCATCAAAA | Start: 3226 |
| Tm | 60.104 | 60.039 | End: 3376 |
| Size | 20 | 20 | 151 |
| Primer 5 details |
|---|
| Sequence | ACGTGGACCCCAAAGTTGTT | CTTTTCGTCAGCTTCCGCAC | Start: 3042 |
| Tm | 60.034 | 60.111 | End: 3206 |
| Size | 20 | 20 | 165 |
(T)20: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTGTCCCAAGTATGGCGGTC | TGGGGAGATTGCTCGAGGTA | Start: 1528 |
| Tm | 60.036 | 60.033 | End: 1730 |
| Size | 20 | 20 | 203 |
| Primer 2 details |
|---|
| Sequence | TCTCGACAACCCTTTGGAGC | CCGTACACACATGGACCCAA | Start: 2001 |
| Tm | 59.965 | 59.964 | End: 2129 |
| Size | 20 | 20 | 129 |
| Primer 3 details |
|---|
| Sequence | ACGTGGACCCCAAAGTTGTT | TTGGGGTCCTTCTTCCAGGA | Start: 3042 |
| Tm | 60.034 | 60.104 | End: 3245 |
| Size | 20 | 20 | 204 |
| Primer 4 details |
|---|
| Sequence | TCCTGGAAGAAGGACCCCAA | CAGCAGCAGCAGCATCAAAA | Start: 3226 |
| Tm | 60.104 | 60.039 | End: 3376 |
| Size | 20 | 20 | 151 |
| Primer 5 details |
|---|
| Sequence | ACGTGGACCCCAAAGTTGTT | CTTTTCGTCAGCTTCCGCAC | Start: 3042 |
| Tm | 60.034 | 60.111 | End: 3206 |
| Size | 20 | 20 | 165 |
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