ssr_primer
Primer Details for TRINITY_DN13721_c1_g10_i1 |
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| (A)23: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGTCAAAGAGGAACGAGGGG | TGCTCCGAACATCCCAAGAC | Start: 978 |
| Tm | 60.036 | 60.036 | End: 1158 |
| Size | 20 | 20 | 181 |
| Primer 2 details |
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| Sequence | GGTCAAAGAGGAACGAGGGG | TGCGCTGGGACTCTGATTTT | Start: 978 |
| Tm | 60.036 | 59.963 | End: 1252 |
| Size | 20 | 20 | 275 |
| Primer 3 details |
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| Sequence | GTCTTGGGATGTTCGGAGCA | TGCGCTGGGACTCTGATTTT | Start: 1139 |
| Tm | 60.036 | 59.963 | End: 1252 |
| Size | 20 | 20 | 114 |
| Primer 4 details |
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| Sequence | GTCTTGGGATGTTCGGAGCA | ATGTGTGATGGGCTCGATGG | Start: 1139 |
| Tm | 60.036 | 60.179 | End: 1374 |
| Size | 20 | 20 | 236 |
| Primer 5 details |
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| Sequence | AAAATCAGAGTCCCAGCGCA | ATGTGTGATGGGCTCGATGG | Start: 1233 |
| Tm | 59.963 | 60.179 | End: 1374 |
| Size | 20 | 20 | 142 |
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