Primer Details for TRINITY_DN13711_c4_g1_i1 |
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| (GA)6aaggaaagaagagagaga(AG)7: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGGAAAATGGAATGTACAAAGCCT | AGGACAGGGTGGACAGATCT | Start: 91 |
| Tm | 59.162 | 59.585 | End: 229 |
| Size | 24 | 20 | 139 |
| Primer 2 details |
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| Sequence | AGATCATGGAGGAGAGTAAACTTCG | AGGACAGGGTGGACAGATCT | Start: 130 |
| Tm | 59.932 | 59.585 | End: 229 |
| Size | 25 | 20 | 100 |
| Primer 3 details |
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| Sequence | TCATGGAGGAGAGTAAACTTCGC | TTAAGGACAGGGTGGACAGA | Start: 133 |
| Tm | 60.119 | 57.591 | End: 232 |
| Size | 23 | 20 | 100 |
| Primer 4 details |
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| Sequence | TGGAAAATGGAATGTACAAAGCC | AGGACAGGGTGGACAGATCT | Start: 91 |
| Tm | 57.854 | 59.585 | End: 229 |
| Size | 23 | 20 | 139 |
| Primer 5 details |
|---|
| Sequence | TGGAAAATGGAATGTACAAAGCCTT | AGGACAGGGTGGACAGATCT | Start: 91 |
| Tm | 59.693 | 59.585 | End: 229 |
| Size | 25 | 20 | 139 |
(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGGAAAATGGAATGTACAAAGCCT | AGGACAGGGTGGACAGATCT | Start: 91 |
| Tm | 59.162 | 59.585 | End: 229 |
| Size | 24 | 20 | 139 |
| Primer 2 details |
|---|
| Sequence | AGATCATGGAGGAGAGTAAACTTCG | AGGACAGGGTGGACAGATCT | Start: 130 |
| Tm | 59.932 | 59.585 | End: 229 |
| Size | 25 | 20 | 100 |
| Primer 3 details |
|---|
| Sequence | TCATGGAGGAGAGTAAACTTCGC | TTAAGGACAGGGTGGACAGA | Start: 133 |
| Tm | 60.119 | 57.591 | End: 232 |
| Size | 23 | 20 | 100 |
| Primer 4 details |
|---|
| Sequence | TGGAAAATGGAATGTACAAAGCC | AGGACAGGGTGGACAGATCT | Start: 91 |
| Tm | 57.854 | 59.585 | End: 229 |
| Size | 23 | 20 | 139 |
| Primer 5 details |
|---|
| Sequence | TGGAAAATGGAATGTACAAAGCCTT | AGGACAGGGTGGACAGATCT | Start: 91 |
| Tm | 59.693 | 59.585 | End: 229 |
| Size | 25 | 20 | 139 |
