ssr_primer
Primer Details for TRINITY_DN13711_c2_g2_i5 |
|---|
| (T)11: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CAACTAGTCCCCAACGACCC | ACTGGTGGGGCAATACCATG | Start: 2565 |
| Tm | 60.037 | 60.033 | End: 2802 |
| Size | 20 | 20 | 238 |
| Primer 2 details |
|---|
| Sequence | CCAACTAGTCCCCAACGACC | ACTGGTGGGGCAATACCATG | Start: 2564 |
| Tm | 60.037 | 60.033 | End: 2802 |
| Size | 20 | 20 | 239 |
| Primer 3 details |
|---|
| Sequence | TGACGCCTCTTTGAAGGTCC | TTGGTCCCTGCCGTTTCTAC | Start: 427 |
| Tm | 59.965 | 59.964 | End: 664 |
| Size | 20 | 20 | 238 |
| Primer 4 details |
|---|
| Sequence | TGCCTGACCGCAACTACATT | GGGAGACAATGCTCGGGAAA | Start: 5481 |
| Tm | 59.964 | 60.035 | End: 5696 |
| Size | 20 | 20 | 216 |
| Primer 5 details |
|---|
| Sequence | TTGCCTGACCGCAACTACAT | GGGAGACAATGCTCGGGAAA | Start: 5480 |
| Tm | 59.964 | 60.035 | End: 5696 |
| Size | 20 | 20 | 217 |
(T)22: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CAACTAGTCCCCAACGACCC | ACTGGTGGGGCAATACCATG | Start: 2565 |
| Tm | 60.037 | 60.033 | End: 2802 |
| Size | 20 | 20 | 238 |
| Primer 2 details |
|---|
| Sequence | CCAACTAGTCCCCAACGACC | ACTGGTGGGGCAATACCATG | Start: 2564 |
| Tm | 60.037 | 60.033 | End: 2802 |
| Size | 20 | 20 | 239 |
| Primer 3 details |
|---|
| Sequence | TGACGCCTCTTTGAAGGTCC | TTGGTCCCTGCCGTTTCTAC | Start: 427 |
| Tm | 59.965 | 59.964 | End: 664 |
| Size | 20 | 20 | 238 |
| Primer 4 details |
|---|
| Sequence | TGCCTGACCGCAACTACATT | GGGAGACAATGCTCGGGAAA | Start: 5481 |
| Tm | 59.964 | 60.035 | End: 5696 |
| Size | 20 | 20 | 216 |
| Primer 5 details |
|---|
| Sequence | TTGCCTGACCGCAACTACAT | GGGAGACAATGCTCGGGAAA | Start: 5480 |
| Tm | 59.964 | 60.035 | End: 5696 |
| Size | 20 | 20 | 217 |
(AT)9: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CAACTAGTCCCCAACGACCC | ACTGGTGGGGCAATACCATG | Start: 2565 |
| Tm | 60.037 | 60.033 | End: 2802 |
| Size | 20 | 20 | 238 |
| Primer 2 details |
|---|
| Sequence | CCAACTAGTCCCCAACGACC | ACTGGTGGGGCAATACCATG | Start: 2564 |
| Tm | 60.037 | 60.033 | End: 2802 |
| Size | 20 | 20 | 239 |
| Primer 3 details |
|---|
| Sequence | TGACGCCTCTTTGAAGGTCC | TTGGTCCCTGCCGTTTCTAC | Start: 427 |
| Tm | 59.965 | 59.964 | End: 664 |
| Size | 20 | 20 | 238 |
| Primer 4 details |
|---|
| Sequence | TGCCTGACCGCAACTACATT | GGGAGACAATGCTCGGGAAA | Start: 5481 |
| Tm | 59.964 | 60.035 | End: 5696 |
| Size | 20 | 20 | 216 |
| Primer 5 details |
|---|
| Sequence | TTGCCTGACCGCAACTACAT | GGGAGACAATGCTCGGGAAA | Start: 5480 |
| Tm | 59.964 | 60.035 | End: 5696 |
| Size | 20 | 20 | 217 |
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