ssr_primers

ssr_primer

Primer Details for TRINITY_DN13688_c0_g2_i3
(T)14: Primer 1 details
Primer Characteristics	Forward Primer	Reverse Primer	Product Details
Sequence	GAACTGGCGGTTGAGGAGAA	CCGGTTAAGGCCAAGGATGT	Start: 2877
Tm	59.965	60.034	End: 3069
Size	20	20	193
Primer 2 details
Sequence	GAACTGGCGGTTGAGGAGAA	GTTCCGGTTAAGGCCAAGGA	Start: 2877
Tm	59.965	59.963	End: 3072
Size	20	20	196
Primer 3 details
Sequence	GAACTGGCGGTTGAGGAGAA	AGTTCCGGTTAAGGCCAAGG	Start: 2877
Tm	59.965	59.962	End: 3073
Size	20	20	197
Primer 4 details
Sequence	ACTGTTGAAGGCGAGGATGG	GGGAGAGTTGTGGCAATGGA	Start: 1042
Tm	60.036	59.962	End: 1209
Size	20	20	168
Primer 5 details
Sequence	ACTGTTGAAGGCGAGGATGG	AGGGGAGAGTTGTGGCAATG	Start: 1042
Tm	60.036	59.961	End: 1211
Size	20	20	170
(A)11: Primer 1 details
Primer Characteristics	Forward Primer	Reverse Primer	Product Details
Sequence	GAACTGGCGGTTGAGGAGAA	CCGGTTAAGGCCAAGGATGT	Start: 2877
Tm	59.965	60.034	End: 3069
Size	20	20	193
Primer 2 details
Sequence	GAACTGGCGGTTGAGGAGAA	GTTCCGGTTAAGGCCAAGGA	Start: 2877
Tm	59.965	59.963	End: 3072
Size	20	20	196
Primer 3 details
Sequence	GAACTGGCGGTTGAGGAGAA	AGTTCCGGTTAAGGCCAAGG	Start: 2877
Tm	59.965	59.962	End: 3073
Size	20	20	197
Primer 4 details
Sequence	ACTGTTGAAGGCGAGGATGG	GGGAGAGTTGTGGCAATGGA	Start: 1042
Tm	60.036	59.962	End: 1209
Size	20	20	168
Primer 5 details
Sequence	ACTGTTGAAGGCGAGGATGG	AGGGGAGAGTTGTGGCAATG	Start: 1042
Tm	60.036	59.961	End: 1211
Size	20	20	170
(TA)9: Primer 1 details
Primer Characteristics	Forward Primer	Reverse Primer	Product Details
Sequence	GAACTGGCGGTTGAGGAGAA	CCGGTTAAGGCCAAGGATGT	Start: 2877
Tm	59.965	60.034	End: 3069
Size	20	20	193
Primer 2 details
Sequence	GAACTGGCGGTTGAGGAGAA	GTTCCGGTTAAGGCCAAGGA	Start: 2877
Tm	59.965	59.963	End: 3072
Size	20	20	196
Primer 3 details
Sequence	GAACTGGCGGTTGAGGAGAA	AGTTCCGGTTAAGGCCAAGG	Start: 2877
Tm	59.965	59.962	End: 3073
Size	20	20	197
Primer 4 details
Sequence	ACTGTTGAAGGCGAGGATGG	GGGAGAGTTGTGGCAATGGA	Start: 1042
Tm	60.036	59.962	End: 1209
Size	20	20	168
Primer 5 details
Sequence	ACTGTTGAAGGCGAGGATGG	AGGGGAGAGTTGTGGCAATG	Start: 1042
Tm	60.036	59.961	End: 1211
Size	20	20	170
(A)11: Primer 1 details
Primer Characteristics	Forward Primer	Reverse Primer	Product Details
Sequence	GAACTGGCGGTTGAGGAGAA	CCGGTTAAGGCCAAGGATGT	Start: 2877
Tm	59.965	60.034	End: 3069
Size	20	20	193
Primer 2 details
Sequence	GAACTGGCGGTTGAGGAGAA	GTTCCGGTTAAGGCCAAGGA	Start: 2877
Tm	59.965	59.963	End: 3072
Size	20	20	196
Primer 3 details
Sequence	GAACTGGCGGTTGAGGAGAA	AGTTCCGGTTAAGGCCAAGG	Start: 2877
Tm	59.965	59.962	End: 3073
Size	20	20	197
Primer 4 details
Sequence	ACTGTTGAAGGCGAGGATGG	GGGAGAGTTGTGGCAATGGA	Start: 1042
Tm	60.036	59.962	End: 1209
Size	20	20	168
Primer 5 details
Sequence	ACTGTTGAAGGCGAGGATGG	AGGGGAGAGTTGTGGCAATG	Start: 1042
Tm	60.036	59.961	End: 1211
Size	20	20	170
(A)13: Primer 1 details
Primer Characteristics	Forward Primer	Reverse Primer	Product Details
Sequence	GAACTGGCGGTTGAGGAGAA	CCGGTTAAGGCCAAGGATGT	Start: 2877
Tm	59.965	60.034	End: 3069
Size	20	20	193
Primer 2 details
Sequence	GAACTGGCGGTTGAGGAGAA	GTTCCGGTTAAGGCCAAGGA	Start: 2877
Tm	59.965	59.963	End: 3072
Size	20	20	196
Primer 3 details
Sequence	GAACTGGCGGTTGAGGAGAA	AGTTCCGGTTAAGGCCAAGG	Start: 2877
Tm	59.965	59.962	End: 3073
Size	20	20	197
Primer 4 details
Sequence	ACTGTTGAAGGCGAGGATGG	GGGAGAGTTGTGGCAATGGA	Start: 1042
Tm	60.036	59.962	End: 1209
Size	20	20	168
Primer 5 details
Sequence	ACTGTTGAAGGCGAGGATGG	AGGGGAGAGTTGTGGCAATG	Start: 1042
Tm	60.036	59.961	End: 1211
Size	20	20	170
(AG)7: Primer 1 details
Primer Characteristics	Forward Primer	Reverse Primer	Product Details
Sequence	GAACTGGCGGTTGAGGAGAA	CCGGTTAAGGCCAAGGATGT	Start: 2877
Tm	59.965	60.034	End: 3069
Size	20	20	193
Primer 2 details
Sequence	GAACTGGCGGTTGAGGAGAA	GTTCCGGTTAAGGCCAAGGA	Start: 2877
Tm	59.965	59.963	End: 3072
Size	20	20	196
Primer 3 details
Sequence	GAACTGGCGGTTGAGGAGAA	AGTTCCGGTTAAGGCCAAGG	Start: 2877
Tm	59.965	59.962	End: 3073
Size	20	20	197
Primer 4 details
Sequence	ACTGTTGAAGGCGAGGATGG	GGGAGAGTTGTGGCAATGGA	Start: 1042
Tm	60.036	59.962	End: 1209
Size	20	20	168
Primer 5 details
Sequence	ACTGTTGAAGGCGAGGATGG	AGGGGAGAGTTGTGGCAATG	Start: 1042
Tm	60.036	59.961	End: 1211
Size	20	20	170

CsExSLDb: Transcriptome based Cucumis sativus Extended Shelf Life Database

Primer Details for TRINITY_DN13688_c0_g2_i3

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