Primer Details for TRINITY_DN13686_c7_g1_i1 | |||
|---|---|---|---|
| (T)10: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCAGTCATTAAATCCATCCTCCCT | TGGATGAGGCTGAAGTTTTGGT | Start: 142 |
| Tm | 59.022 | 60.158 | End: 389 |
| Size | 24 | 22 | 248 |
| Primer 2 details | |||
| Sequence | GTCAGTCATTAAATCCATCCTCCC | TGGATGAGGCTGAAGTTTTGGT | Start: 141 |
| Tm | 58.933 | 60.158 | End: 389 |
| Size | 24 | 22 | 249 |
| Primer 3 details | |||
| Sequence | GTCAGTCATTAAATCCATCCTCCCT | TGGATGAGGCTGAAGTTTTGGT | Start: 141 |
| Tm | 60.163 | 60.158 | End: 389 |
| Size | 25 | 22 | 249 |
| Primer 4 details | |||
| Sequence | AGTCAGTCATTAAATCCATCCTCCC | TGGATGAGGCTGAAGTTTTGGT | Start: 140 |
| Tm | 60.163 | 60.158 | End: 389 |
| Size | 25 | 22 | 250 |
| Primer 5 details | |||
| Sequence | TCAGTCATTAAATCCATCCTCCC | TGGATGAGGCTGAAGTTTTGGT | Start: 142 |
| Tm | 57.69 | 60.158 | End: 389 |
| Size | 23 | 22 | 248 |
