Primer Details for TRINITY_DN13686_c6_g1_i1 |
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| (A)17: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACCTAACCCTTAAGCATACAAACA | CCCGGTGCGGCCTATATTTA | Start: 49 |
| Tm | 58.117 | 59.678 | End: 164 |
| Size | 24 | 20 | 116 |
| Primer 2 details |
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| Sequence | AACCTAACCCTTAAGCATACAAACA | CCCGGTGCGGCCTATATTTA | Start: 48 |
| Tm | 58.691 | 59.678 | End: 164 |
| Size | 25 | 20 | 117 |
| Primer 3 details |
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| Sequence | CACTATATAAATATAGGCCGCACCG | CACTGGTGCAGGACTTTGAC | Start: 138 |
| Tm | 59.31 | 59.053 | End: 237 |
| Size | 25 | 20 | 100 |
| Primer 4 details |
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| Sequence | ACCTAACCCTTAAGCATACAAACA | ACTGGTGCAGGACTTTGACT | Start: 49 |
| Tm | 58.117 | 59.16 | End: 236 |
| Size | 24 | 20 | 188 |
| Primer 5 details |
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| Sequence | ACCTAACCCTTAAGCATACAAACAT | CCCGGTGCGGCCTATATTTA | Start: 49 |
| Tm | 58.51 | 59.678 | End: 164 |
| Size | 25 | 20 | 116 |
