ssr_primer
Primer Details for TRINITY_DN13686_c3_g5_i6 |
|---|
| (T)12: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTCAAAGGGATGAGCGCCAT | AGGAGGGCTAGAGATTGGCA | Start: 241 |
| Tm | 60.034 | 60.031 | End: 376 |
| Size | 20 | 20 | 136 |
| Primer 2 details |
|---|
| Sequence | ATTCAAAGGGATGAGCGCCA | AGGAGGGCTAGAGATTGGCA | Start: 240 |
| Tm | 60.034 | 60.031 | End: 376 |
| Size | 20 | 20 | 137 |
| Primer 3 details |
|---|
| Sequence | CCCCTTTCGACGTCCATTCA | AGGAGGGCTAGAGATTGGCA | Start: 225 |
| Tm | 60.037 | 60.031 | End: 376 |
| Size | 20 | 20 | 152 |
| Primer 4 details |
|---|
| Sequence | AGCCTAACCACCAAGCATCC | TGCATTGTGATGAGGGGGTC | Start: 3463 |
| Tm | 60.034 | 60.034 | End: 3607 |
| Size | 20 | 20 | 145 |
| Primer 5 details |
|---|
| Sequence | CCTGGGTGTAAAGCTGGGAG | ATCATGGCCAAGAAGCGGAA | Start: 3956 |
| Tm | 60.035 | 60.034 | End: 4067 |
| Size | 20 | 20 | 112 |
(TA)8aaaatataagaaacaatctttcgttggttgtttgacttatagttatatt(TA)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTCAAAGGGATGAGCGCCAT | AGGAGGGCTAGAGATTGGCA | Start: 241 |
| Tm | 60.034 | 60.031 | End: 376 |
| Size | 20 | 20 | 136 |
| Primer 2 details |
|---|
| Sequence | ATTCAAAGGGATGAGCGCCA | AGGAGGGCTAGAGATTGGCA | Start: 240 |
| Tm | 60.034 | 60.031 | End: 376 |
| Size | 20 | 20 | 137 |
| Primer 3 details |
|---|
| Sequence | CCCCTTTCGACGTCCATTCA | AGGAGGGCTAGAGATTGGCA | Start: 225 |
| Tm | 60.037 | 60.031 | End: 376 |
| Size | 20 | 20 | 152 |
| Primer 4 details |
|---|
| Sequence | AGCCTAACCACCAAGCATCC | TGCATTGTGATGAGGGGGTC | Start: 3463 |
| Tm | 60.034 | 60.034 | End: 3607 |
| Size | 20 | 20 | 145 |
| Primer 5 details |
|---|
| Sequence | CCTGGGTGTAAAGCTGGGAG | ATCATGGCCAAGAAGCGGAA | Start: 3956 |
| Tm | 60.035 | 60.034 | End: 4067 |
| Size | 20 | 20 | 112 |
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