ssr_primer
Primer Details for TRINITY_DN13670_c1_g1_i1 |
|---|
| (A)20: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCGACAAGTAGGGGGAGGAG | TCCGATTCCCTTCACCAACG | Start: 949 |
| Tm | 60.033 | 60.037 | End: 1139 |
| Size | 20 | 20 | 191 |
| Primer 2 details |
|---|
| Sequence | CGTTGGTGAAGGGAATCGGA | CCGGTGGTTACTTTCACGGA | Start: 1120 |
| Tm | 60.037 | 59.966 | End: 1389 |
| Size | 20 | 20 | 270 |
| Primer 3 details |
|---|
| Sequence | TTCCAGAGAGAAGCCTCCGA | TCCGATTCCCTTCACCAACG | Start: 977 |
| Tm | 59.96 | 60.037 | End: 1139 |
| Size | 20 | 20 | 163 |
| Primer 4 details |
|---|
| Sequence | ACAAGTAGGGGGAGGAGTCC | TCCGATTCCCTTCACCAACG | Start: 952 |
| Tm | 59.957 | 60.037 | End: 1139 |
| Size | 20 | 20 | 188 |
| Primer 5 details |
|---|
| Sequence | CGTTAAGCCATTCGCCTTCG | TCTAGAAACGGCATCGACGG | Start: 294 |
| Tm | 59.974 | 59.9 | End: 492 |
| Size | 20 | 20 | 199 |
(AG)9: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCGACAAGTAGGGGGAGGAG | TCCGATTCCCTTCACCAACG | Start: 949 |
| Tm | 60.033 | 60.037 | End: 1139 |
| Size | 20 | 20 | 191 |
| Primer 2 details |
|---|
| Sequence | CGTTGGTGAAGGGAATCGGA | CCGGTGGTTACTTTCACGGA | Start: 1120 |
| Tm | 60.037 | 59.966 | End: 1389 |
| Size | 20 | 20 | 270 |
| Primer 3 details |
|---|
| Sequence | TTCCAGAGAGAAGCCTCCGA | TCCGATTCCCTTCACCAACG | Start: 977 |
| Tm | 59.96 | 60.037 | End: 1139 |
| Size | 20 | 20 | 163 |
| Primer 4 details |
|---|
| Sequence | ACAAGTAGGGGGAGGAGTCC | TCCGATTCCCTTCACCAACG | Start: 952 |
| Tm | 59.957 | 60.037 | End: 1139 |
| Size | 20 | 20 | 188 |
| Primer 5 details |
|---|
| Sequence | CGTTAAGCCATTCGCCTTCG | TCTAGAAACGGCATCGACGG | Start: 294 |
| Tm | 59.974 | 59.9 | End: 492 |
| Size | 20 | 20 | 199 |
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