ssr_primer
Primer Details for TRINITY_DN13663_c2_g2_i14 |
|---|
| (TTA)5: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTTGCCGCTGGAGTGATGAT | AAACCCCCACACAGAAGCAA | Start: 4995 |
| Tm | 60.035 | 60.033 | End: 5237 |
| Size | 20 | 20 | 243 |
| Primer 2 details |
|---|
| Sequence | CCCCATCTCCTTCAAACGCT | GTTGGACACCATGGGAGAGG | Start: 125 |
| Tm | 60.035 | 60.035 | End: 251 |
| Size | 20 | 20 | 127 |
| Primer 3 details |
|---|
| Sequence | GGCATGTTGGTCTGGGTGTA | AAACCCCCACACAGAAGCAA | Start: 5114 |
| Tm | 59.962 | 60.033 | End: 5237 |
| Size | 20 | 20 | 124 |
| Primer 4 details |
|---|
| Sequence | TCATGTGGTGGGAGTGCTTC | CCTGCAGAATAAGGGTGCCA | Start: 3128 |
| Tm | 59.963 | 60.034 | End: 3373 |
| Size | 20 | 20 | 246 |
| Primer 5 details |
|---|
| Sequence | ATGTGGTGGGAGTGCTTCTG | CCTGCAGAATAAGGGTGCCA | Start: 3130 |
| Tm | 59.963 | 60.034 | End: 3373 |
| Size | 20 | 20 | 244 |
(T)27: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTTGCCGCTGGAGTGATGAT | AAACCCCCACACAGAAGCAA | Start: 4995 |
| Tm | 60.035 | 60.033 | End: 5237 |
| Size | 20 | 20 | 243 |
| Primer 2 details |
|---|
| Sequence | CCCCATCTCCTTCAAACGCT | GTTGGACACCATGGGAGAGG | Start: 125 |
| Tm | 60.035 | 60.035 | End: 251 |
| Size | 20 | 20 | 127 |
| Primer 3 details |
|---|
| Sequence | GGCATGTTGGTCTGGGTGTA | AAACCCCCACACAGAAGCAA | Start: 5114 |
| Tm | 59.962 | 60.033 | End: 5237 |
| Size | 20 | 20 | 124 |
| Primer 4 details |
|---|
| Sequence | TCATGTGGTGGGAGTGCTTC | CCTGCAGAATAAGGGTGCCA | Start: 3128 |
| Tm | 59.963 | 60.034 | End: 3373 |
| Size | 20 | 20 | 246 |
| Primer 5 details |
|---|
| Sequence | ATGTGGTGGGAGTGCTTCTG | CCTGCAGAATAAGGGTGCCA | Start: 3130 |
| Tm | 59.963 | 60.034 | End: 3373 |
| Size | 20 | 20 | 244 |
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