ssr_primer
Primer Details for TRINITY_DN13653_c4_g6_i2 |
|---|
| (T)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGGCATGTCTTTTTCACCGG | ACCATACCTTCACTGCCTGC | Start: 3831 |
| Tm | 60.11 | 60.035 | End: 3931 |
| Size | 20 | 20 | 101 |
| Primer 2 details |
|---|
| Sequence | ATCCCAACCACCACCATCAC | TGCTTTTCCTCCCGTTTGGT | Start: 356 |
| Tm | 59.96 | 60.106 | End: 547 |
| Size | 20 | 20 | 192 |
| Primer 3 details |
|---|
| Sequence | CTTGACAATGGCTGCTGCTG | TCGACAAGAGCTGCAGAGTG | Start: 1242 |
| Tm | 60.109 | 60.039 | End: 1490 |
| Size | 20 | 20 | 249 |
| Primer 4 details |
|---|
| Sequence | GACAATGGCTGCTGCTGTTC | TCGACAAGAGCTGCAGAGTG | Start: 1245 |
| Tm | 60.109 | 60.039 | End: 1490 |
| Size | 20 | 20 | 246 |
| Primer 5 details |
|---|
| Sequence | ACCAAACGGGAGGAAAAGCA | AAACTCCTGGGTCAGCTGTG | Start: 528 |
| Tm | 60.106 | 59.891 | End: 653 |
| Size | 20 | 20 | 126 |
(AT)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGGCATGTCTTTTTCACCGG | ACCATACCTTCACTGCCTGC | Start: 3831 |
| Tm | 60.11 | 60.035 | End: 3931 |
| Size | 20 | 20 | 101 |
| Primer 2 details |
|---|
| Sequence | ATCCCAACCACCACCATCAC | TGCTTTTCCTCCCGTTTGGT | Start: 356 |
| Tm | 59.96 | 60.106 | End: 547 |
| Size | 20 | 20 | 192 |
| Primer 3 details |
|---|
| Sequence | CTTGACAATGGCTGCTGCTG | TCGACAAGAGCTGCAGAGTG | Start: 1242 |
| Tm | 60.109 | 60.039 | End: 1490 |
| Size | 20 | 20 | 249 |
| Primer 4 details |
|---|
| Sequence | GACAATGGCTGCTGCTGTTC | TCGACAAGAGCTGCAGAGTG | Start: 1245 |
| Tm | 60.109 | 60.039 | End: 1490 |
| Size | 20 | 20 | 246 |
| Primer 5 details |
|---|
| Sequence | ACCAAACGGGAGGAAAAGCA | AAACTCCTGGGTCAGCTGTG | Start: 528 |
| Tm | 60.106 | 59.891 | End: 653 |
| Size | 20 | 20 | 126 |
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