Primer Details for TRINITY_DN13653_c1_g1_i1 |
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| (GA)6: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGCTAATAAGGAAAATCACTCTGGG | TGGGTGAATTGGTAGTTTAAGAGA | Start: 84 |
| Tm | 58.878 | 57.557 | End: 216 |
| Size | 25 | 24 | 133 |
| Primer 2 details |
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| Sequence | GCTAATAAGGAAAATCACTCTGGGT | TGGGTGAATTGGTAGTTTAAGAGA | Start: 85 |
| Tm | 58.877 | 57.557 | End: 216 |
| Size | 25 | 24 | 132 |
| Primer 3 details |
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| Sequence | TGCTAATAAGGAAAATCACTCTGGGT | TGGGTGAATTGGTAGTTTAAGAGA | Start: 84 |
| Tm | 60.297 | 57.557 | End: 216 |
| Size | 26 | 24 | 133 |
| Primer 4 details |
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| Sequence | TGCTAATAAGGAAAATCACTCTGGG | TGGGTGAATTGGTAGTTTAAGAGAA | Start: 84 |
| Tm | 58.878 | 58.156 | End: 216 |
| Size | 25 | 25 | 133 |
| Primer 5 details |
|---|
| Sequence | TGCTAATAAGGAAAATCACTCTGGG | TTGGGTGAATTGGTAGTTTAAGAGA | Start: 84 |
| Tm | 58.878 | 58.156 | End: 217 |
| Size | 25 | 25 | 134 |
