ssr_primer
Primer Details for TRINITY_DN13651_c5_g7_i6 |
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| (T)20: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACGAGTCACTGCGATGTGTT | GCTCGGTACCACCAGTCAAA | Start: 484 |
| Tm | 59.968 | 59.966 | End: 680 |
| Size | 20 | 20 | 197 |
| Primer 2 details |
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| Sequence | TTCTCGTGGGGATCAACAGC | TCACCACCGCTAACTTCCAC | Start: 981 |
| Tm | 60.036 | 59.966 | End: 1195 |
| Size | 20 | 20 | 215 |
| Primer 3 details |
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| Sequence | CTCCCGTCTTCTCTTCGTCG | AACACATCGCAGTGACTCGT | Start: 285 |
| Tm | 59.903 | 59.968 | End: 503 |
| Size | 20 | 20 | 219 |
| Primer 4 details |
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| Sequence | TTTGACTGGTGGTACCGAGC | TCATCGACAGTGACCTTCGC | Start: 661 |
| Tm | 59.966 | 60.109 | End: 915 |
| Size | 20 | 20 | 255 |
| Primer 5 details |
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| Sequence | CTCACGAGTCACTGCGATGT | GCTCGGTACCACCAGTCAAA | Start: 481 |
| Tm | 60.11 | 59.966 | End: 680 |
| Size | 20 | 20 | 200 |
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