ssr_primer
Primer Details for TRINITY_DN13651_c5_g5_i5 |
|---|
| (T)13: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CACGTGGCCGGAACAAAAAT | AATGGTGGCTCGTGAAGGAG | Start: 379 |
| Tm | 59.969 | 60.036 | End: 551 |
| Size | 20 | 20 | 173 |
| Primer 2 details |
|---|
| Sequence | CTCCTTCACGAGCCACCATT | ACAAGTCAGCGTTCGTGCTA | Start: 532 |
| Tm | 60.036 | 59.968 | End: 729 |
| Size | 20 | 20 | 198 |
| Primer 3 details |
|---|
| Sequence | CTCCTTCACGAGCCACCATT | CTGGTGGAAGAAGGCGGAAT | Start: 532 |
| Tm | 60.036 | 60.035 | End: 673 |
| Size | 20 | 20 | 142 |
| Primer 4 details |
|---|
| Sequence | CGTTGGAGTTCCGGACATGA | CAGGCGGCTATGGAGATCAG | Start: 983 |
| Tm | 60.038 | 60.038 | End: 1222 |
| Size | 20 | 20 | 240 |
| Primer 5 details |
|---|
| Sequence | CGTTGGAGTTCCGGACATGA | GGCGGCTATGGAGATCAGAC | Start: 983 |
| Tm | 60.038 | 60.038 | End: 1220 |
| Size | 20 | 20 | 238 |
(TC)6: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CACGTGGCCGGAACAAAAAT | AATGGTGGCTCGTGAAGGAG | Start: 379 |
| Tm | 59.969 | 60.036 | End: 551 |
| Size | 20 | 20 | 173 |
| Primer 2 details |
|---|
| Sequence | CTCCTTCACGAGCCACCATT | ACAAGTCAGCGTTCGTGCTA | Start: 532 |
| Tm | 60.036 | 59.968 | End: 729 |
| Size | 20 | 20 | 198 |
| Primer 3 details |
|---|
| Sequence | CTCCTTCACGAGCCACCATT | CTGGTGGAAGAAGGCGGAAT | Start: 532 |
| Tm | 60.036 | 60.035 | End: 673 |
| Size | 20 | 20 | 142 |
| Primer 4 details |
|---|
| Sequence | CGTTGGAGTTCCGGACATGA | CAGGCGGCTATGGAGATCAG | Start: 983 |
| Tm | 60.038 | 60.038 | End: 1222 |
| Size | 20 | 20 | 240 |
| Primer 5 details |
|---|
| Sequence | CGTTGGAGTTCCGGACATGA | GGCGGCTATGGAGATCAGAC | Start: 983 |
| Tm | 60.038 | 60.038 | End: 1220 |
| Size | 20 | 20 | 238 |
(T)11: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CACGTGGCCGGAACAAAAAT | AATGGTGGCTCGTGAAGGAG | Start: 379 |
| Tm | 59.969 | 60.036 | End: 551 |
| Size | 20 | 20 | 173 |
| Primer 2 details |
|---|
| Sequence | CTCCTTCACGAGCCACCATT | ACAAGTCAGCGTTCGTGCTA | Start: 532 |
| Tm | 60.036 | 59.968 | End: 729 |
| Size | 20 | 20 | 198 |
| Primer 3 details |
|---|
| Sequence | CTCCTTCACGAGCCACCATT | CTGGTGGAAGAAGGCGGAAT | Start: 532 |
| Tm | 60.036 | 60.035 | End: 673 |
| Size | 20 | 20 | 142 |
| Primer 4 details |
|---|
| Sequence | CGTTGGAGTTCCGGACATGA | CAGGCGGCTATGGAGATCAG | Start: 983 |
| Tm | 60.038 | 60.038 | End: 1222 |
| Size | 20 | 20 | 240 |
| Primer 5 details |
|---|
| Sequence | CGTTGGAGTTCCGGACATGA | GGCGGCTATGGAGATCAGAC | Start: 983 |
| Tm | 60.038 | 60.038 | End: 1220 |
| Size | 20 | 20 | 238 |
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