ssr_primer
Primer Details for TRINITY_DN13649_c1_g1_i1 |
|---|
| (A)39: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ATTGCAGTCACCTCGCTCAA | CCGAAAGACCACCAGCATCT | Start: 269 |
| Tm | 59.965 | 60.036 | End: 392 |
| Size | 20 | 20 | 124 |
| Primer 2 details |
|---|
| Sequence | GGCAAGTCCATTTGGCTGTG | TTGAGCGAGGTGACTGCAAT | Start: 180 |
| Tm | 60.038 | 59.965 | End: 288 |
| Size | 20 | 20 | 109 |
| Primer 3 details |
|---|
| Sequence | GGCAAGTCCATTTGGCTGTG | CCGAAAGACCACCAGCATCT | Start: 180 |
| Tm | 60.038 | 60.036 | End: 392 |
| Size | 20 | 20 | 213 |
| Primer 4 details |
|---|
| Sequence | CTCGTTTCTTTGGGGGCTCT | CACCCTGGAGATGTTGCAGT | Start: 1017 |
| Tm | 59.963 | 59.963 | End: 1140 |
| Size | 20 | 20 | 124 |
| Primer 5 details |
|---|
| Sequence | ATTGCAGTCACCTCGCTCAA | TGGAACCGAAAGACCACCAG | Start: 269 |
| Tm | 59.965 | 59.892 | End: 397 |
| Size | 20 | 20 | 129 |
(A)17caaaagggaaacaagtgacaagaatcagaaagc(T)11: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ATTGCAGTCACCTCGCTCAA | CCGAAAGACCACCAGCATCT | Start: 269 |
| Tm | 59.965 | 60.036 | End: 392 |
| Size | 20 | 20 | 124 |
| Primer 2 details |
|---|
| Sequence | GGCAAGTCCATTTGGCTGTG | TTGAGCGAGGTGACTGCAAT | Start: 180 |
| Tm | 60.038 | 59.965 | End: 288 |
| Size | 20 | 20 | 109 |
| Primer 3 details |
|---|
| Sequence | GGCAAGTCCATTTGGCTGTG | CCGAAAGACCACCAGCATCT | Start: 180 |
| Tm | 60.038 | 60.036 | End: 392 |
| Size | 20 | 20 | 213 |
| Primer 4 details |
|---|
| Sequence | CTCGTTTCTTTGGGGGCTCT | CACCCTGGAGATGTTGCAGT | Start: 1017 |
| Tm | 59.963 | 59.963 | End: 1140 |
| Size | 20 | 20 | 124 |
| Primer 5 details |
|---|
| Sequence | ATTGCAGTCACCTCGCTCAA | TGGAACCGAAAGACCACCAG | Start: 269 |
| Tm | 59.965 | 59.892 | End: 397 |
| Size | 20 | 20 | 129 |
(AG)14: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ATTGCAGTCACCTCGCTCAA | CCGAAAGACCACCAGCATCT | Start: 269 |
| Tm | 59.965 | 60.036 | End: 392 |
| Size | 20 | 20 | 124 |
| Primer 2 details |
|---|
| Sequence | GGCAAGTCCATTTGGCTGTG | TTGAGCGAGGTGACTGCAAT | Start: 180 |
| Tm | 60.038 | 59.965 | End: 288 |
| Size | 20 | 20 | 109 |
| Primer 3 details |
|---|
| Sequence | GGCAAGTCCATTTGGCTGTG | CCGAAAGACCACCAGCATCT | Start: 180 |
| Tm | 60.038 | 60.036 | End: 392 |
| Size | 20 | 20 | 213 |
| Primer 4 details |
|---|
| Sequence | CTCGTTTCTTTGGGGGCTCT | CACCCTGGAGATGTTGCAGT | Start: 1017 |
| Tm | 59.963 | 59.963 | End: 1140 |
| Size | 20 | 20 | 124 |
| Primer 5 details |
|---|
| Sequence | ATTGCAGTCACCTCGCTCAA | TGGAACCGAAAGACCACCAG | Start: 269 |
| Tm | 59.965 | 59.892 | End: 397 |
| Size | 20 | 20 | 129 |
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