ssr_primer
Primer Details for TRINITY_DN13634_c1_g1_i6 |
|---|
| (T)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCCTTAGCTCTCCTCCCACC | TGTTCAAGGAGATGGTGGCC | Start: 822 |
| Tm | 60.032 | 59.962 | End: 970 |
| Size | 20 | 20 | 149 |
| Primer 2 details |
|---|
| Sequence | ACCAACGAGACGCCTTGTAG | TGAGGATACACAGCAGGGGA | Start: 5310 |
| Tm | 60.039 | 59.957 | End: 5437 |
| Size | 20 | 20 | 128 |
| Primer 3 details |
|---|
| Sequence | CCCAACAGCACGTAAAGCAC | TGAGGATACACAGCAGGGGA | Start: 5276 |
| Tm | 60.04 | 59.957 | End: 5437 |
| Size | 20 | 20 | 162 |
| Primer 4 details |
|---|
| Sequence | CACCCAACAGCACGTAAAGC | TGAGGATACACAGCAGGGGA | Start: 5274 |
| Tm | 60.04 | 59.957 | End: 5437 |
| Size | 20 | 20 | 164 |
| Primer 5 details |
|---|
| Sequence | CTCCCACCACATCGTAGCTC | TGTTCAAGGAGATGGTGGCC | Start: 834 |
| Tm | 59.897 | 59.962 | End: 970 |
| Size | 20 | 20 | 137 |
(A)12: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCCTTAGCTCTCCTCCCACC | TGTTCAAGGAGATGGTGGCC | Start: 822 |
| Tm | 60.032 | 59.962 | End: 970 |
| Size | 20 | 20 | 149 |
| Primer 2 details |
|---|
| Sequence | ACCAACGAGACGCCTTGTAG | TGAGGATACACAGCAGGGGA | Start: 5310 |
| Tm | 60.039 | 59.957 | End: 5437 |
| Size | 20 | 20 | 128 |
| Primer 3 details |
|---|
| Sequence | CCCAACAGCACGTAAAGCAC | TGAGGATACACAGCAGGGGA | Start: 5276 |
| Tm | 60.04 | 59.957 | End: 5437 |
| Size | 20 | 20 | 162 |
| Primer 4 details |
|---|
| Sequence | CACCCAACAGCACGTAAAGC | TGAGGATACACAGCAGGGGA | Start: 5274 |
| Tm | 60.04 | 59.957 | End: 5437 |
| Size | 20 | 20 | 164 |
| Primer 5 details |
|---|
| Sequence | CTCCCACCACATCGTAGCTC | TGTTCAAGGAGATGGTGGCC | Start: 834 |
| Tm | 59.897 | 59.962 | End: 970 |
| Size | 20 | 20 | 137 |
(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCCTTAGCTCTCCTCCCACC | TGTTCAAGGAGATGGTGGCC | Start: 822 |
| Tm | 60.032 | 59.962 | End: 970 |
| Size | 20 | 20 | 149 |
| Primer 2 details |
|---|
| Sequence | ACCAACGAGACGCCTTGTAG | TGAGGATACACAGCAGGGGA | Start: 5310 |
| Tm | 60.039 | 59.957 | End: 5437 |
| Size | 20 | 20 | 128 |
| Primer 3 details |
|---|
| Sequence | CCCAACAGCACGTAAAGCAC | TGAGGATACACAGCAGGGGA | Start: 5276 |
| Tm | 60.04 | 59.957 | End: 5437 |
| Size | 20 | 20 | 162 |
| Primer 4 details |
|---|
| Sequence | CACCCAACAGCACGTAAAGC | TGAGGATACACAGCAGGGGA | Start: 5274 |
| Tm | 60.04 | 59.957 | End: 5437 |
| Size | 20 | 20 | 164 |
| Primer 5 details |
|---|
| Sequence | CTCCCACCACATCGTAGCTC | TGTTCAAGGAGATGGTGGCC | Start: 834 |
| Tm | 59.897 | 59.962 | End: 970 |
| Size | 20 | 20 | 137 |
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