ssr_primer
Primer Details for TRINITY_DN13633_c1_g1_i25 |
|---|
| (T)11: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCCACACGCTTCATTGTCGA | CAACAGTGTTGCTTCCGTGG | Start: 1029 |
| Tm | 59.967 | 59.971 | End: 1203 |
| Size | 20 | 20 | 175 |
| Primer 2 details |
|---|
| Sequence | CCCTTAGCTCCTCGCAACAA | CAACAGTGTTGCTTCCGTGG | Start: 986 |
| Tm | 60.036 | 59.971 | End: 1203 |
| Size | 20 | 20 | 218 |
| Primer 3 details |
|---|
| Sequence | TCCACACGCTTCATTGTCGA | GGGTGCTCAACAATGGAGGA | Start: 1029 |
| Tm | 59.967 | 59.962 | End: 1254 |
| Size | 20 | 20 | 226 |
| Primer 4 details |
|---|
| Sequence | GCAGGATATCACCACCGGAG | GCAGGTTATTGTGCGTTGCA | Start: 3893 |
| Tm | 59.966 | 60.04 | End: 4057 |
| Size | 20 | 20 | 165 |
| Primer 5 details |
|---|
| Sequence | GCAGGATATCACCACCGGAG | TGCAGGTTATTGTGCGTTGC | Start: 3893 |
| Tm | 59.966 | 60.04 | End: 4058 |
| Size | 20 | 20 | 166 |
(AG)7aaatgagggaggtgtgag(GAA)6: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCCACACGCTTCATTGTCGA | CAACAGTGTTGCTTCCGTGG | Start: 1029 |
| Tm | 59.967 | 59.971 | End: 1203 |
| Size | 20 | 20 | 175 |
| Primer 2 details |
|---|
| Sequence | CCCTTAGCTCCTCGCAACAA | CAACAGTGTTGCTTCCGTGG | Start: 986 |
| Tm | 60.036 | 59.971 | End: 1203 |
| Size | 20 | 20 | 218 |
| Primer 3 details |
|---|
| Sequence | TCCACACGCTTCATTGTCGA | GGGTGCTCAACAATGGAGGA | Start: 1029 |
| Tm | 59.967 | 59.962 | End: 1254 |
| Size | 20 | 20 | 226 |
| Primer 4 details |
|---|
| Sequence | GCAGGATATCACCACCGGAG | GCAGGTTATTGTGCGTTGCA | Start: 3893 |
| Tm | 59.966 | 60.04 | End: 4057 |
| Size | 20 | 20 | 165 |
| Primer 5 details |
|---|
| Sequence | GCAGGATATCACCACCGGAG | TGCAGGTTATTGTGCGTTGC | Start: 3893 |
| Tm | 59.966 | 60.04 | End: 4058 |
| Size | 20 | 20 | 166 |
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