ssr_primer
Primer Details for TRINITY_DN13629_c1_g1_i1 |
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| (T)20: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGCTTTACAAGAATGTCTTTGCA | TCGGTGGACGCTATATCCCA | Start: 13 |
| Tm | 57.487 | 60.179 | End: 178 |
| Size | 23 | 20 | 166 |
| Primer 2 details |
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| Sequence | TGCTTTACAAGAATGTCTTTGCAA | TCGGTGGACGCTATATCCCA | Start: 13 |
| Tm | 58.1 | 60.179 | End: 178 |
| Size | 24 | 20 | 166 |
| Primer 3 details |
|---|
| Sequence | TTGCTTTACAAGAATGTCTTTGCA | TCGGTGGACGCTATATCCCA | Start: 12 |
| Tm | 58.1 | 60.179 | End: 178 |
| Size | 24 | 20 | 167 |
| Primer 4 details |
|---|
| Sequence | TGCTTTACAAGAATGTCTTTGCA | CGGTGGACGCTATATCCCAA | Start: 13 |
| Tm | 57.487 | 59.323 | End: 177 |
| Size | 23 | 20 | 165 |
| Primer 5 details |
|---|
| Sequence | TGCTTTACAAGAATGTCTTTGCA | GGACGCTATATCCCAAGCCA | Start: 13 |
| Tm | 57.487 | 59.315 | End: 173 |
| Size | 23 | 20 | 161 |
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