ssr_primer
Primer Details for TRINITY_DN13628_c1_g8_i1 |
|---|
| (T)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTTGCAGCTGAAGACCTCCA | ATCCGTTTGTGGGTGGCTAG | Start: 54 |
| Tm | 59.964 | 60.035 | End: 258 |
| Size | 20 | 20 | 205 |
| Primer 2 details |
|---|
| Sequence | AGGAGAGAGGAGAGAGGGGA | ATCCGTTTGTGGGTGGCTAG | Start: 124 |
| Tm | 59.953 | 60.035 | End: 258 |
| Size | 20 | 20 | 135 |
| Primer 3 details |
|---|
| Sequence | CTAGCCACCCACAAACGGAT | GATCAAAGGTGAGACGGGGG | Start: 239 |
| Tm | 60.035 | 60.108 | End: 446 |
| Size | 20 | 20 | 208 |
| Primer 4 details |
|---|
| Sequence | CTAGCCACCCACAAACGGAT | AGGTGAGACGGGGGAGTTTA | Start: 239 |
| Tm | 60.035 | 59.884 | End: 440 |
| Size | 20 | 20 | 202 |
| Primer 5 details |
|---|
| Sequence | TTTCTGCCGATTCCACTGCA | CCCAGCAATGACGCCAAAAA | Start: 996 |
| Tm | 60.251 | 59.967 | End: 1142 |
| Size | 20 | 20 | 147 |
(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTTGCAGCTGAAGACCTCCA | ATCCGTTTGTGGGTGGCTAG | Start: 54 |
| Tm | 59.964 | 60.035 | End: 258 |
| Size | 20 | 20 | 205 |
| Primer 2 details |
|---|
| Sequence | AGGAGAGAGGAGAGAGGGGA | ATCCGTTTGTGGGTGGCTAG | Start: 124 |
| Tm | 59.953 | 60.035 | End: 258 |
| Size | 20 | 20 | 135 |
| Primer 3 details |
|---|
| Sequence | CTAGCCACCCACAAACGGAT | GATCAAAGGTGAGACGGGGG | Start: 239 |
| Tm | 60.035 | 60.108 | End: 446 |
| Size | 20 | 20 | 208 |
| Primer 4 details |
|---|
| Sequence | CTAGCCACCCACAAACGGAT | AGGTGAGACGGGGGAGTTTA | Start: 239 |
| Tm | 60.035 | 59.884 | End: 440 |
| Size | 20 | 20 | 202 |
| Primer 5 details |
|---|
| Sequence | TTTCTGCCGATTCCACTGCA | CCCAGCAATGACGCCAAAAA | Start: 996 |
| Tm | 60.251 | 59.967 | End: 1142 |
| Size | 20 | 20 | 147 |
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