ssr_primer
Primer Details for TRINITY_DN13622_c0_g1_i3 |
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| (AAT)6: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AAACCTGCCGATCCAGAAGG | CCGTCTTCTTCGAAGCCACT | Start: 1537 |
| Tm | 60.035 | 60.038 | End: 1649 |
| Size | 20 | 20 | 113 |
| Primer 2 details |
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| Sequence | AAACCTGCCGATCCAGAAGG | GAGATGCGGCTCTCAAGGTT | Start: 1537 |
| Tm | 60.035 | 60.108 | End: 1760 |
| Size | 20 | 20 | 224 |
| Primer 3 details |
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| Sequence | TCCCGCTAGAAGATGGGGAA | CTAAAGGGATGGGGATGGCC | Start: 550 |
| Tm | 60.032 | 59.887 | End: 806 |
| Size | 20 | 20 | 257 |
| Primer 4 details |
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| Sequence | TTCCCGCTAGAAGATGGGGA | CTAAAGGGATGGGGATGGCC | Start: 549 |
| Tm | 60.032 | 59.887 | End: 806 |
| Size | 20 | 20 | 258 |
| Primer 5 details |
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| Sequence | AGTGGCTTCGAAGAAGACGG | GAGATGCGGCTCTCAAGGTT | Start: 1630 |
| Tm | 60.038 | 60.108 | End: 1760 |
| Size | 20 | 20 | 131 |
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