Primer Details for TRINITY_DN13596_c3_g1_i1 | |||
|---|---|---|---|
| (T)10(A)13: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCACCTGATGGAGAATCGGT | ATCCACTATGTGCTTGAAGAGT | Start: 63 |
| Tm | 59.821 | 57.167 | End: 186 |
| Size | 20 | 22 | 124 |
| Primer 2 details | |||
| Sequence | GGCACCTGATGGAGAATCGG | ATCCACTATGTGCTTGAAGAGT | Start: 62 |
| Tm | 60.534 | 57.167 | End: 186 |
| Size | 20 | 22 | 125 |
| Primer 3 details | |||
| Sequence | GCACCTGATGGAGAATCGGT | TCCACTATGTGCTTGAAGAGTAAGT | Start: 63 |
| Tm | 59.821 | 59.754 | End: 185 |
| Size | 20 | 25 | 123 |
| Primer 4 details | |||
| Sequence | GCACCTGATGGAGAATCGGT | CCACTATGTGCTTGAAGAGTAAGT | Start: 63 |
| Tm | 59.821 | 58.517 | End: 184 |
| Size | 20 | 24 | 122 |
| Primer 5 details | |||
| Sequence | GGCACCTGATGGAGAATCGG | TCCACTATGTGCTTGAAGAGTAAGT | Start: 62 |
| Tm | 60.534 | 59.754 | End: 185 |
| Size | 20 | 25 | 124 |
