ssr_primer
Primer Details for TRINITY_DN13576_c2_g2_i1 |
|---|
| (T)14: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACCCATGAGAAGCCCCAAAG | ACTGATTTTGCCCACCCACA | Start: 235 |
| Tm | 59.96 | 60.106 | End: 481 |
| Size | 20 | 20 | 247 |
| Primer 2 details |
|---|
| Sequence | ACCCATGAGAAGCCCCAAAG | TCTACCACCCTCCAACTCGT | Start: 235 |
| Tm | 59.96 | 59.886 | End: 458 |
| Size | 20 | 20 | 224 |
| Primer 3 details |
|---|
| Sequence | ACCCATGAGAAGCCCCAAAG | GCTTCCTACCGCCCCAAATA | Start: 235 |
| Tm | 59.96 | 59.818 | End: 407 |
| Size | 20 | 20 | 173 |
| Primer 4 details |
|---|
| Sequence | TGTGGGTGGGCAAAATCAGT | AGGGAGTTGAGGGCCTACAA | Start: 462 |
| Tm | 60.106 | 60.179 | End: 710 |
| Size | 20 | 20 | 249 |
| Primer 5 details |
|---|
| Sequence | ACGAGTTGGAGGGTGGTAGA | AGGGAGTTGAGGGCCTACAA | Start: 439 |
| Tm | 59.886 | 60.179 | End: 710 |
| Size | 20 | 20 | 272 |
(AAC)7: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACCCATGAGAAGCCCCAAAG | ACTGATTTTGCCCACCCACA | Start: 235 |
| Tm | 59.96 | 60.106 | End: 481 |
| Size | 20 | 20 | 247 |
| Primer 2 details |
|---|
| Sequence | ACCCATGAGAAGCCCCAAAG | TCTACCACCCTCCAACTCGT | Start: 235 |
| Tm | 59.96 | 59.886 | End: 458 |
| Size | 20 | 20 | 224 |
| Primer 3 details |
|---|
| Sequence | ACCCATGAGAAGCCCCAAAG | GCTTCCTACCGCCCCAAATA | Start: 235 |
| Tm | 59.96 | 59.818 | End: 407 |
| Size | 20 | 20 | 173 |
| Primer 4 details |
|---|
| Sequence | TGTGGGTGGGCAAAATCAGT | AGGGAGTTGAGGGCCTACAA | Start: 462 |
| Tm | 60.106 | 60.179 | End: 710 |
| Size | 20 | 20 | 249 |
| Primer 5 details |
|---|
| Sequence | ACGAGTTGGAGGGTGGTAGA | AGGGAGTTGAGGGCCTACAA | Start: 439 |
| Tm | 59.886 | 60.179 | End: 710 |
| Size | 20 | 20 | 272 |
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