ssr_primer
Primer Details for TRINITY_DN13576_c1_g1_i2 |
|---|
| (T)13: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AAGAGACCACCGTCGTGTTC | ACGAGGGAGTCACTGACGTA | Start: 60 |
| Tm | 59.968 | 59.964 | End: 219 |
| Size | 20 | 20 | 160 |
| Primer 2 details |
|---|
| Sequence | GCGACGATGAGGCCTTTAGA | ACGAGGGAGTCACTGACGTA | Start: 35 |
| Tm | 59.898 | 59.964 | End: 219 |
| Size | 20 | 20 | 185 |
| Primer 3 details |
|---|
| Sequence | AAGAGACCACCGTCGTGTTC | GAAAACTGCCGCCATGAGTG | Start: 60 |
| Tm | 59.968 | 60.11 | End: 302 |
| Size | 20 | 20 | 243 |
| Primer 4 details |
|---|
| Sequence | TACGTCAGTGACTCCCTCGT | CCGTCCTAAGCCGCCTTTTA | Start: 200 |
| Tm | 59.964 | 60.108 | End: 424 |
| Size | 20 | 20 | 225 |
| Primer 5 details |
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| Sequence | CTACTGCTTTCGCCTCCACA | CCGTCCTAAGCCGCCTTTTA | Start: 166 |
| Tm | 60.037 | 60.108 | End: 424 |
| Size | 20 | 20 | 259 |
(TTC)5: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AAGAGACCACCGTCGTGTTC | ACGAGGGAGTCACTGACGTA | Start: 60 |
| Tm | 59.968 | 59.964 | End: 219 |
| Size | 20 | 20 | 160 |
| Primer 2 details |
|---|
| Sequence | GCGACGATGAGGCCTTTAGA | ACGAGGGAGTCACTGACGTA | Start: 35 |
| Tm | 59.898 | 59.964 | End: 219 |
| Size | 20 | 20 | 185 |
| Primer 3 details |
|---|
| Sequence | AAGAGACCACCGTCGTGTTC | GAAAACTGCCGCCATGAGTG | Start: 60 |
| Tm | 59.968 | 60.11 | End: 302 |
| Size | 20 | 20 | 243 |
| Primer 4 details |
|---|
| Sequence | TACGTCAGTGACTCCCTCGT | CCGTCCTAAGCCGCCTTTTA | Start: 200 |
| Tm | 59.964 | 60.108 | End: 424 |
| Size | 20 | 20 | 225 |
| Primer 5 details |
|---|
| Sequence | CTACTGCTTTCGCCTCCACA | CCGTCCTAAGCCGCCTTTTA | Start: 166 |
| Tm | 60.037 | 60.108 | End: 424 |
| Size | 20 | 20 | 259 |
(TA)12: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AAGAGACCACCGTCGTGTTC | ACGAGGGAGTCACTGACGTA | Start: 60 |
| Tm | 59.968 | 59.964 | End: 219 |
| Size | 20 | 20 | 160 |
| Primer 2 details |
|---|
| Sequence | GCGACGATGAGGCCTTTAGA | ACGAGGGAGTCACTGACGTA | Start: 35 |
| Tm | 59.898 | 59.964 | End: 219 |
| Size | 20 | 20 | 185 |
| Primer 3 details |
|---|
| Sequence | AAGAGACCACCGTCGTGTTC | GAAAACTGCCGCCATGAGTG | Start: 60 |
| Tm | 59.968 | 60.11 | End: 302 |
| Size | 20 | 20 | 243 |
| Primer 4 details |
|---|
| Sequence | TACGTCAGTGACTCCCTCGT | CCGTCCTAAGCCGCCTTTTA | Start: 200 |
| Tm | 59.964 | 60.108 | End: 424 |
| Size | 20 | 20 | 225 |
| Primer 5 details |
|---|
| Sequence | CTACTGCTTTCGCCTCCACA | CCGTCCTAAGCCGCCTTTTA | Start: 166 |
| Tm | 60.037 | 60.108 | End: 424 |
| Size | 20 | 20 | 259 |
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