ssr_primer
Primer Details for TRINITY_DN13561_c1_g2_i6 |
|---|
| (T)15: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CACAGTTGGTCTTACGGCCT | CATCTTCCCCATGCCACTGT | Start: 923 |
| Tm | 59.965 | 60.034 | End: 1182 |
| Size | 20 | 20 | 260 |
| Primer 2 details |
|---|
| Sequence | GAGGCACAAAGGGAGTCCTC | CATCTTCCCCATGCCACTGT | Start: 1058 |
| Tm | 60.036 | 60.034 | End: 1182 |
| Size | 20 | 20 | 125 |
| Primer 3 details |
|---|
| Sequence | ACAGTGGCATGGGGAAGATG | ATTCTCACGGGAACTGGCTG | Start: 1163 |
| Tm | 60.034 | 60.036 | End: 1381 |
| Size | 20 | 20 | 219 |
| Primer 4 details |
|---|
| Sequence | CGGCCTCTGGTGATGTCTTT | CATCTTCCCCATGCCACTGT | Start: 937 |
| Tm | 60.036 | 60.034 | End: 1182 |
| Size | 20 | 20 | 246 |
| Primer 5 details |
|---|
| Sequence | TGACCCCGTATCGTGTTGTG | AGGGCAAACCAAACCTCACA | Start: 1612 |
| Tm | 60.038 | 60.033 | End: 1747 |
| Size | 20 | 20 | 136 |
(T)12: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CACAGTTGGTCTTACGGCCT | CATCTTCCCCATGCCACTGT | Start: 923 |
| Tm | 59.965 | 60.034 | End: 1182 |
| Size | 20 | 20 | 260 |
| Primer 2 details |
|---|
| Sequence | GAGGCACAAAGGGAGTCCTC | CATCTTCCCCATGCCACTGT | Start: 1058 |
| Tm | 60.036 | 60.034 | End: 1182 |
| Size | 20 | 20 | 125 |
| Primer 3 details |
|---|
| Sequence | ACAGTGGCATGGGGAAGATG | ATTCTCACGGGAACTGGCTG | Start: 1163 |
| Tm | 60.034 | 60.036 | End: 1381 |
| Size | 20 | 20 | 219 |
| Primer 4 details |
|---|
| Sequence | CGGCCTCTGGTGATGTCTTT | CATCTTCCCCATGCCACTGT | Start: 937 |
| Tm | 60.036 | 60.034 | End: 1182 |
| Size | 20 | 20 | 246 |
| Primer 5 details |
|---|
| Sequence | TGACCCCGTATCGTGTTGTG | AGGGCAAACCAAACCTCACA | Start: 1612 |
| Tm | 60.038 | 60.033 | End: 1747 |
| Size | 20 | 20 | 136 |
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