ssr_primer
Primer Details for TRINITY_DN13552_c2_g1_i3 |
|---|
| (T)20: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AACCATATCCCGACCCTCCA | CTGGCTCTGGCTCAAGTTCA | Start: 459 |
| Tm | 60.031 | 59.964 | End: 617 |
| Size | 20 | 20 | 159 |
| Primer 2 details |
|---|
| Sequence | AACCATATCCCGACCCTCCA | GGAGTCGGAGTTGGACTTGG | Start: 459 |
| Tm | 60.031 | 60.037 | End: 702 |
| Size | 20 | 20 | 244 |
| Primer 3 details |
|---|
| Sequence | AACCATATCCCGACCCTCCA | ATTGGTGGAAGTGGCTCTGG | Start: 459 |
| Tm | 60.031 | 59.961 | End: 663 |
| Size | 20 | 20 | 205 |
| Primer 4 details |
|---|
| Sequence | CGAACCTCCACCATATCCCG | CTGGCTCTGGCTCAAGTTCA | Start: 439 |
| Tm | 59.966 | 59.964 | End: 617 |
| Size | 20 | 20 | 179 |
| Primer 5 details |
|---|
| Sequence | TCCTTTCTTGCCACTCGACC | GCTTTAACACAGTCCCCCGA | Start: 722 |
| Tm | 59.965 | 59.964 | End: 855 |
| Size | 20 | 20 | 134 |
(T)17: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AACCATATCCCGACCCTCCA | CTGGCTCTGGCTCAAGTTCA | Start: 459 |
| Tm | 60.031 | 59.964 | End: 617 |
| Size | 20 | 20 | 159 |
| Primer 2 details |
|---|
| Sequence | AACCATATCCCGACCCTCCA | GGAGTCGGAGTTGGACTTGG | Start: 459 |
| Tm | 60.031 | 60.037 | End: 702 |
| Size | 20 | 20 | 244 |
| Primer 3 details |
|---|
| Sequence | AACCATATCCCGACCCTCCA | ATTGGTGGAAGTGGCTCTGG | Start: 459 |
| Tm | 60.031 | 59.961 | End: 663 |
| Size | 20 | 20 | 205 |
| Primer 4 details |
|---|
| Sequence | CGAACCTCCACCATATCCCG | CTGGCTCTGGCTCAAGTTCA | Start: 439 |
| Tm | 59.966 | 59.964 | End: 617 |
| Size | 20 | 20 | 179 |
| Primer 5 details |
|---|
| Sequence | TCCTTTCTTGCCACTCGACC | GCTTTAACACAGTCCCCCGA | Start: 722 |
| Tm | 59.965 | 59.964 | End: 855 |
| Size | 20 | 20 | 134 |
(GAGCCA)5: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AACCATATCCCGACCCTCCA | CTGGCTCTGGCTCAAGTTCA | Start: 459 |
| Tm | 60.031 | 59.964 | End: 617 |
| Size | 20 | 20 | 159 |
| Primer 2 details |
|---|
| Sequence | AACCATATCCCGACCCTCCA | GGAGTCGGAGTTGGACTTGG | Start: 459 |
| Tm | 60.031 | 60.037 | End: 702 |
| Size | 20 | 20 | 244 |
| Primer 3 details |
|---|
| Sequence | AACCATATCCCGACCCTCCA | ATTGGTGGAAGTGGCTCTGG | Start: 459 |
| Tm | 60.031 | 59.961 | End: 663 |
| Size | 20 | 20 | 205 |
| Primer 4 details |
|---|
| Sequence | CGAACCTCCACCATATCCCG | CTGGCTCTGGCTCAAGTTCA | Start: 439 |
| Tm | 59.966 | 59.964 | End: 617 |
| Size | 20 | 20 | 179 |
| Primer 5 details |
|---|
| Sequence | TCCTTTCTTGCCACTCGACC | GCTTTAACACAGTCCCCCGA | Start: 722 |
| Tm | 59.965 | 59.964 | End: 855 |
| Size | 20 | 20 | 134 |
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