Primer Details for TRINITY_DN13547_c4_g2_i1 |
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| (A)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TAGTGCAGCAACTCCCACTG | ACACTTGTATCTTTGGGAATGTTGT | Start: 42 |
| Tm | 59.965 | 59.167 | End: 201 |
| Size | 20 | 25 | 160 |
| Primer 2 details |
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| Sequence | CAGCAACTCCCACTGTGACA | ACACTTGTATCTTTGGGAATGTTGT | Start: 47 |
| Tm | 60.179 | 59.167 | End: 201 |
| Size | 20 | 25 | 155 |
| Primer 3 details |
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| Sequence | GGATAGTGCAGCAACTCCCA | ACACTTGTATCTTTGGGAATGTTGT | Start: 39 |
| Tm | 59.747 | 59.167 | End: 201 |
| Size | 20 | 25 | 163 |
| Primer 4 details |
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| Sequence | GGGATAGTGCAGCAACTCCC | ACACTTGTATCTTTGGGAATGTTGT | Start: 38 |
| Tm | 60.465 | 59.167 | End: 201 |
| Size | 20 | 25 | 164 |
| Primer 5 details |
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| Sequence | TAGTGCAGCAACTCCCACTG | ACACTTGTATCTTTGGGAATGTTGTT | Start: 42 |
| Tm | 59.965 | 59.676 | End: 201 |
| Size | 20 | 26 | 160 |
